Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(®)/ET(® )Recombination

BACKGROUND: Introducing point mutations into bacterial chromosomes is important for further progress in studies relying on functional genomics, systems- and synthetic biology, and for metabolic engineering. For many investigations, chromosomal systems are required rather than artificial plasmid base...

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Detalles Bibliográficos
Autores principales: Heermann, Ralf, Zeppenfeld, Tim, Jung, Kirsten
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Technical Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373285/
https://www.ncbi.nlm.nih.gov/pubmed/18435843
http://dx.doi.org/10.1186/1475-2859-7-14

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373285/
https://www.ncbi.nlm.nih.gov/pubmed/18435843
http://dx.doi.org/10.1186/1475-2859-7-14

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