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Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red(®)/ET(® )Recombination
BACKGROUND: Introducing point mutations into bacterial chromosomes is important for further progress in studies relying on functional genomics, systems- and synthetic biology, and for metabolic engineering. For many investigations, chromosomal systems are required rather than artificial plasmid base...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373285/ https://www.ncbi.nlm.nih.gov/pubmed/18435843 http://dx.doi.org/10.1186/1475-2859-7-14 |