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Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells

To determine how extracellular signal–regulated kinases (ERK) 1/2 promote mammary tumorigenesis, we examined the real-time behavior of cells in an organotypic culture of the mammary glandular epithelium. Inducible activation of ERK1/2 in mature acini elicits cell motility and disrupts epithelial arc...

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Detalles Bibliográficos
Autores principales: Pearson, Gray W., Hunter, Tony
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373504/
https://www.ncbi.nlm.nih.gov/pubmed/18166657
http://dx.doi.org/10.1083/jcb.200706099
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author Pearson, Gray W.
Hunter, Tony
author_facet Pearson, Gray W.
Hunter, Tony
author_sort Pearson, Gray W.
collection PubMed
description To determine how extracellular signal–regulated kinases (ERK) 1/2 promote mammary tumorigenesis, we examined the real-time behavior of cells in an organotypic culture of the mammary glandular epithelium. Inducible activation of ERK1/2 in mature acini elicits cell motility and disrupts epithelial architecture in a manner that is reminiscent of ductal carcinoma in situ; however, motile cells do not invade through the basement membrane and branching morphogenesis does not take place. ERK1/2-induced motility causes cells to move both within the cell monolayer that contacts the basement membrane surrounding the acinus and through the luminal space of the acinus. E-cadherin expression is reduced after ERK1/2 activation, but motility does not involve an epithelial–mesenchymal transition. Cell motility and the disruption of epithelial architecture require a Rho kinase– and myosin light chain kinase–dependent increase in the phosphorylation of myosin light chain 2. Our results identify a new mechanism for the disruption of architecture in epithelial acini and suggest that ERK1/2 can promote noninvasive motility in preinvasive mammary tumors.
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spelling pubmed-23735042008-06-30 Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells Pearson, Gray W. Hunter, Tony J Cell Biol Research Articles To determine how extracellular signal–regulated kinases (ERK) 1/2 promote mammary tumorigenesis, we examined the real-time behavior of cells in an organotypic culture of the mammary glandular epithelium. Inducible activation of ERK1/2 in mature acini elicits cell motility and disrupts epithelial architecture in a manner that is reminiscent of ductal carcinoma in situ; however, motile cells do not invade through the basement membrane and branching morphogenesis does not take place. ERK1/2-induced motility causes cells to move both within the cell monolayer that contacts the basement membrane surrounding the acinus and through the luminal space of the acinus. E-cadherin expression is reduced after ERK1/2 activation, but motility does not involve an epithelial–mesenchymal transition. Cell motility and the disruption of epithelial architecture require a Rho kinase– and myosin light chain kinase–dependent increase in the phosphorylation of myosin light chain 2. Our results identify a new mechanism for the disruption of architecture in epithelial acini and suggest that ERK1/2 can promote noninvasive motility in preinvasive mammary tumors. The Rockefeller University Press 2007-12-31 /pmc/articles/PMC2373504/ /pubmed/18166657 http://dx.doi.org/10.1083/jcb.200706099 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Pearson, Gray W.
Hunter, Tony
Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells
title Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells
title_full Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells
title_fullStr Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells
title_full_unstemmed Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells
title_short Real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells
title_sort real-time imaging reveals that noninvasive mammary epithelial acini can contain motile cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2373504/
https://www.ncbi.nlm.nih.gov/pubmed/18166657
http://dx.doi.org/10.1083/jcb.200706099
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