Hypermethylation of CpG islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells

We have sequenced the promoter region of the murine asparagine synthetase gene and examined its methylation profile in the CpG islands of L-asparaginase-sensitive 6C3HED cells (asparagine auxotrophs) and resistant variants (prototrophs). In the former, complete methylation of the CpG island is corre...

Descripción completa

Detalles Bibliográficos
Autores principales: Peng, H, Shen, N, Qian, L, Sun, X-L, Koduru, P, Goodwin, L O, Issa, J-P, Broome, J D
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2001
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375082/
https://www.ncbi.nlm.nih.gov/pubmed/11556848
http://dx.doi.org/10.1054/bjoc.2001.2000
_version_ 1782154573223297024
author Peng, H
Shen, N
Qian, L
Sun, X-L
Koduru, P
Goodwin, L O
Issa, J-P
Broome, J D
author_facet Peng, H
Shen, N
Qian, L
Sun, X-L
Koduru, P
Goodwin, L O
Issa, J-P
Broome, J D
author_sort Peng, H
collection PubMed
description We have sequenced the promoter region of the murine asparagine synthetase gene and examined its methylation profile in the CpG islands of L-asparaginase-sensitive 6C3HED cells (asparagine auxotrophs) and resistant variants (prototrophs). In the former, complete methylation of the CpG island is correlated with failure of expression of mRNA: cells of the latter possess both methylated and unmethylated alleles, as do cells of the intrinsically asparagine-independent lines L1210 and EL4. A similar phenomenon was seen in normal splenic cells of adult mice. This was age related: no methylation was found in weanlings, but up to 45% of gene copies in animals 18 weeks or older were methylated. It was also tissue related, with methylation occurring rarely in liver cells. The relationship of these changes to oncogenesis is considered. http://www.bjcancer.com © 2001 Cancer Research Campaignhttp://www.bjcancer.com
format Text
id pubmed-2375082
institution National Center for Biotechnology Information
language English
publishDate 2001
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-23750822009-09-10 Hypermethylation of CpG islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells Peng, H Shen, N Qian, L Sun, X-L Koduru, P Goodwin, L O Issa, J-P Broome, J D Br J Cancer Regular Article We have sequenced the promoter region of the murine asparagine synthetase gene and examined its methylation profile in the CpG islands of L-asparaginase-sensitive 6C3HED cells (asparagine auxotrophs) and resistant variants (prototrophs). In the former, complete methylation of the CpG island is correlated with failure of expression of mRNA: cells of the latter possess both methylated and unmethylated alleles, as do cells of the intrinsically asparagine-independent lines L1210 and EL4. A similar phenomenon was seen in normal splenic cells of adult mice. This was age related: no methylation was found in weanlings, but up to 45% of gene copies in animals 18 weeks or older were methylated. It was also tissue related, with methylation occurring rarely in liver cells. The relationship of these changes to oncogenesis is considered. http://www.bjcancer.com © 2001 Cancer Research Campaignhttp://www.bjcancer.com Nature Publishing Group 2001-09 /pmc/articles/PMC2375082/ /pubmed/11556848 http://dx.doi.org/10.1054/bjoc.2001.2000 Text en Copyright © 2001 Cancer Research Campaign https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Regular Article
Peng, H
Shen, N
Qian, L
Sun, X-L
Koduru, P
Goodwin, L O
Issa, J-P
Broome, J D
Hypermethylation of CpG islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells
title Hypermethylation of CpG islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells
title_full Hypermethylation of CpG islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells
title_fullStr Hypermethylation of CpG islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells
title_full_unstemmed Hypermethylation of CpG islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells
title_short Hypermethylation of CpG islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. Partial methylation in normal cells
title_sort hypermethylation of cpg islands in the mouse asparagine synthetase gene: relationship to asparaginase sensitivity in lymphoma cells. partial methylation in normal cells
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2375082/
https://www.ncbi.nlm.nih.gov/pubmed/11556848
http://dx.doi.org/10.1054/bjoc.2001.2000
work_keys_str_mv AT pengh hypermethylationofcpgislandsinthemouseasparaginesynthetasegenerelationshiptoasparaginasesensitivityinlymphomacellspartialmethylationinnormalcells
AT shenn hypermethylationofcpgislandsinthemouseasparaginesynthetasegenerelationshiptoasparaginasesensitivityinlymphomacellspartialmethylationinnormalcells
AT qianl hypermethylationofcpgislandsinthemouseasparaginesynthetasegenerelationshiptoasparaginasesensitivityinlymphomacellspartialmethylationinnormalcells
AT sunxl hypermethylationofcpgislandsinthemouseasparaginesynthetasegenerelationshiptoasparaginasesensitivityinlymphomacellspartialmethylationinnormalcells
AT kodurup hypermethylationofcpgislandsinthemouseasparaginesynthetasegenerelationshiptoasparaginasesensitivityinlymphomacellspartialmethylationinnormalcells
AT goodwinlo hypermethylationofcpgislandsinthemouseasparaginesynthetasegenerelationshiptoasparaginasesensitivityinlymphomacellspartialmethylationinnormalcells
AT issajp hypermethylationofcpgislandsinthemouseasparaginesynthetasegenerelationshiptoasparaginasesensitivityinlymphomacellspartialmethylationinnormalcells
AT broomejd hypermethylationofcpgislandsinthemouseasparaginesynthetasegenerelationshiptoasparaginasesensitivityinlymphomacellspartialmethylationinnormalcells

Ejemplares similares