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A systems biology approach to analyse amplification in the JAK2-STAT5 signalling pathway
BACKGROUND: The amplification of signals, defined as an increase in the intensity of a signal through networks of intracellular reactions, is considered one of the essential properties in many cell signalling pathways. Despite of the apparent importance of signal amplification, there have been few a...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386439/ https://www.ncbi.nlm.nih.gov/pubmed/18439261 http://dx.doi.org/10.1186/1752-0509-2-38 |
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author | Vera, Julio Bachmann, Julie Pfeifer, Andrea C Becker, Verena Hormiga, Jose A Darias, Nestor V Torres Timmer, Jens Klingmüller, Ursula Wolkenhauer, Olaf |
author_facet | Vera, Julio Bachmann, Julie Pfeifer, Andrea C Becker, Verena Hormiga, Jose A Darias, Nestor V Torres Timmer, Jens Klingmüller, Ursula Wolkenhauer, Olaf |
author_sort | Vera, Julio |
collection | PubMed |
description | BACKGROUND: The amplification of signals, defined as an increase in the intensity of a signal through networks of intracellular reactions, is considered one of the essential properties in many cell signalling pathways. Despite of the apparent importance of signal amplification, there have been few attempts to formalise this concept. RESULTS: In this work we investigate the amplification and responsiveness of the JAK2-STAT5 pathway using a kinetic model. The recruitment of EpoR to the plasma membrane, activation by Epo, and deactivation of the EpoR/JAK2 complex are considered as well as the activation and nucleocytoplasmic shuttling of STAT5. Using qualitative biological knowledge, we first establish the structure of a general power-law model. We then generate a family of models from which we select suitable candidates. The parameter values of the model are estimated from experimental quantitative time-course data. The final model, whether it is conventional model with fixed predefined integer kinetic orders or a model with variable non-integer kinetic orders, is selected on the basis of a good agreement between simulations and the experimental data. The model is used to analyse the responsiveness and amplification properties of the pathway with sustained, transient, and oscillatory stimulation. CONCLUSION: The selected kinetic model predicts that the system acts as an amplifier with maximum amplification and sensitivity for input signals whose intensity match physiological values for Epo concentration and with duration in the range of one to 100 minutes. The response of the system reaches saturation for more intense and longer stimulation with Epo. We hypothesise that these properties of the system directly relate to the saturation of Epo receptor activation, its low recruitment to the plasma membrane and intense deactivation as predicted by the model. |
format | Text |
id | pubmed-2386439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-23864392008-05-16 A systems biology approach to analyse amplification in the JAK2-STAT5 signalling pathway Vera, Julio Bachmann, Julie Pfeifer, Andrea C Becker, Verena Hormiga, Jose A Darias, Nestor V Torres Timmer, Jens Klingmüller, Ursula Wolkenhauer, Olaf BMC Syst Biol Research Article BACKGROUND: The amplification of signals, defined as an increase in the intensity of a signal through networks of intracellular reactions, is considered one of the essential properties in many cell signalling pathways. Despite of the apparent importance of signal amplification, there have been few attempts to formalise this concept. RESULTS: In this work we investigate the amplification and responsiveness of the JAK2-STAT5 pathway using a kinetic model. The recruitment of EpoR to the plasma membrane, activation by Epo, and deactivation of the EpoR/JAK2 complex are considered as well as the activation and nucleocytoplasmic shuttling of STAT5. Using qualitative biological knowledge, we first establish the structure of a general power-law model. We then generate a family of models from which we select suitable candidates. The parameter values of the model are estimated from experimental quantitative time-course data. The final model, whether it is conventional model with fixed predefined integer kinetic orders or a model with variable non-integer kinetic orders, is selected on the basis of a good agreement between simulations and the experimental data. The model is used to analyse the responsiveness and amplification properties of the pathway with sustained, transient, and oscillatory stimulation. CONCLUSION: The selected kinetic model predicts that the system acts as an amplifier with maximum amplification and sensitivity for input signals whose intensity match physiological values for Epo concentration and with duration in the range of one to 100 minutes. The response of the system reaches saturation for more intense and longer stimulation with Epo. We hypothesise that these properties of the system directly relate to the saturation of Epo receptor activation, its low recruitment to the plasma membrane and intense deactivation as predicted by the model. BioMed Central 2008-04-25 /pmc/articles/PMC2386439/ /pubmed/18439261 http://dx.doi.org/10.1186/1752-0509-2-38 Text en Copyright © 2008 Vera et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Vera, Julio Bachmann, Julie Pfeifer, Andrea C Becker, Verena Hormiga, Jose A Darias, Nestor V Torres Timmer, Jens Klingmüller, Ursula Wolkenhauer, Olaf A systems biology approach to analyse amplification in the JAK2-STAT5 signalling pathway |
title | A systems biology approach to analyse amplification in the JAK2-STAT5 signalling pathway |
title_full | A systems biology approach to analyse amplification in the JAK2-STAT5 signalling pathway |
title_fullStr | A systems biology approach to analyse amplification in the JAK2-STAT5 signalling pathway |
title_full_unstemmed | A systems biology approach to analyse amplification in the JAK2-STAT5 signalling pathway |
title_short | A systems biology approach to analyse amplification in the JAK2-STAT5 signalling pathway |
title_sort | systems biology approach to analyse amplification in the jak2-stat5 signalling pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386439/ https://www.ncbi.nlm.nih.gov/pubmed/18439261 http://dx.doi.org/10.1186/1752-0509-2-38 |
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