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In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis
The antineoplastic potential of a stable monomeric Au(II) complex with hematoporphyrin IX (Hp), namely [Au(II)Hp(−2H).(H(2)O)(2)], was investigated in a panel of tumor cell lines. The complex exhibits strong cytotoxicity, whereby the leukaemia- and lymphoma-derived cell lines are more sensitive, wit...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386889/ https://www.ncbi.nlm.nih.gov/pubmed/18497870 http://dx.doi.org/10.1155/2008/367471 |
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author | Momekov, Georgi Ferdinandov, Dilyan Konstantinov, Spiro Arpadjan, Sonja Tsekova, Daniela Gencheva, Galina Bontchev, Panayot R. Karaivanova, Margarita |
author_facet | Momekov, Georgi Ferdinandov, Dilyan Konstantinov, Spiro Arpadjan, Sonja Tsekova, Daniela Gencheva, Galina Bontchev, Panayot R. Karaivanova, Margarita |
author_sort | Momekov, Georgi |
collection | PubMed |
description | The antineoplastic potential of a stable monomeric Au(II) complex with hematoporphyrin IX (Hp), namely [Au(II)Hp(−2H).(H(2)O)(2)], was investigated in a panel of tumor cell lines. The complex exhibits strong cytotoxicity, whereby the leukaemia- and lymphoma-derived cell lines are more sensitive, with IC(50) values comparable to those of the reference anticancer drug cisplatin. In contrast, the solid tumor models are more sensitive to the platinum drug. A comparative assessment of both agents against the human kidney cell line 293T has shown that [Au(II)Hp(−2H).(H(2)O)(2)] is less cytotoxic. The gold complex induces oligonucleosomal DNA fragmentation in tumour cells following 24-hour treatment and hence its cytotoxic effect is at least partly mediated by induction of apoptotic cell death. A prominent intracellular gold accumulation was detected after treating tumor cells with [Au(II)Hp(−2H).(H(2)O)(2)] which shows that its putative pharmacological targets are readily accessible after a short incubation period. |
format | Text |
id | pubmed-2386889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-23868892008-05-22 In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis Momekov, Georgi Ferdinandov, Dilyan Konstantinov, Spiro Arpadjan, Sonja Tsekova, Daniela Gencheva, Galina Bontchev, Panayot R. Karaivanova, Margarita Bioinorg Chem Appl Research Article The antineoplastic potential of a stable monomeric Au(II) complex with hematoporphyrin IX (Hp), namely [Au(II)Hp(−2H).(H(2)O)(2)], was investigated in a panel of tumor cell lines. The complex exhibits strong cytotoxicity, whereby the leukaemia- and lymphoma-derived cell lines are more sensitive, with IC(50) values comparable to those of the reference anticancer drug cisplatin. In contrast, the solid tumor models are more sensitive to the platinum drug. A comparative assessment of both agents against the human kidney cell line 293T has shown that [Au(II)Hp(−2H).(H(2)O)(2)] is less cytotoxic. The gold complex induces oligonucleosomal DNA fragmentation in tumour cells following 24-hour treatment and hence its cytotoxic effect is at least partly mediated by induction of apoptotic cell death. A prominent intracellular gold accumulation was detected after treating tumor cells with [Au(II)Hp(−2H).(H(2)O)(2)] which shows that its putative pharmacological targets are readily accessible after a short incubation period. Hindawi Publishing Corporation 2008 2008-05-15 /pmc/articles/PMC2386889/ /pubmed/18497870 http://dx.doi.org/10.1155/2008/367471 Text en Copyright © 2008 Georgi Momekov et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Momekov, Georgi Ferdinandov, Dilyan Konstantinov, Spiro Arpadjan, Sonja Tsekova, Daniela Gencheva, Galina Bontchev, Panayot R. Karaivanova, Margarita In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis |
title | In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis |
title_full | In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis |
title_fullStr | In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis |
title_full_unstemmed | In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis |
title_short | In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis |
title_sort | in vitro evaluation of a stable monomeric gold(ii) complex with hematoporphyrin ix: cytotoxicity against tumor and kidney cells, cellular accumulation, and induction of apoptosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386889/ https://www.ncbi.nlm.nih.gov/pubmed/18497870 http://dx.doi.org/10.1155/2008/367471 |
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