Cargando…

In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis

The antineoplastic potential of a stable monomeric Au(II) complex with hematoporphyrin IX (Hp), namely [Au(II)Hp(−2H).(H(2)O)(2)], was investigated in a panel of tumor cell lines. The complex exhibits strong cytotoxicity, whereby the leukaemia- and lymphoma-derived cell lines are more sensitive, wit...

Descripción completa

Detalles Bibliográficos
Autores principales: Momekov, Georgi, Ferdinandov, Dilyan, Konstantinov, Spiro, Arpadjan, Sonja, Tsekova, Daniela, Gencheva, Galina, Bontchev, Panayot R., Karaivanova, Margarita
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386889/
https://www.ncbi.nlm.nih.gov/pubmed/18497870
http://dx.doi.org/10.1155/2008/367471
_version_ 1782155280567500800
author Momekov, Georgi
Ferdinandov, Dilyan
Konstantinov, Spiro
Arpadjan, Sonja
Tsekova, Daniela
Gencheva, Galina
Bontchev, Panayot R.
Karaivanova, Margarita
author_facet Momekov, Georgi
Ferdinandov, Dilyan
Konstantinov, Spiro
Arpadjan, Sonja
Tsekova, Daniela
Gencheva, Galina
Bontchev, Panayot R.
Karaivanova, Margarita
author_sort Momekov, Georgi
collection PubMed
description The antineoplastic potential of a stable monomeric Au(II) complex with hematoporphyrin IX (Hp), namely [Au(II)Hp(−2H).(H(2)O)(2)], was investigated in a panel of tumor cell lines. The complex exhibits strong cytotoxicity, whereby the leukaemia- and lymphoma-derived cell lines are more sensitive, with IC(50) values comparable to those of the reference anticancer drug cisplatin. In contrast, the solid tumor models are more sensitive to the platinum drug. A comparative assessment of both agents against the human kidney cell line 293T has shown that [Au(II)Hp(−2H).(H(2)O)(2)] is less cytotoxic. The gold complex induces oligonucleosomal DNA fragmentation in tumour cells following 24-hour treatment and hence its cytotoxic effect is at least partly mediated by induction of apoptotic cell death. A prominent intracellular gold accumulation was detected after treating tumor cells with [Au(II)Hp(−2H).(H(2)O)(2)] which shows that its putative pharmacological targets are readily accessible after a short incubation period.
format Text
id pubmed-2386889
institution National Center for Biotechnology Information
language English
publishDate 2008
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-23868892008-05-22 In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis Momekov, Georgi Ferdinandov, Dilyan Konstantinov, Spiro Arpadjan, Sonja Tsekova, Daniela Gencheva, Galina Bontchev, Panayot R. Karaivanova, Margarita Bioinorg Chem Appl Research Article The antineoplastic potential of a stable monomeric Au(II) complex with hematoporphyrin IX (Hp), namely [Au(II)Hp(−2H).(H(2)O)(2)], was investigated in a panel of tumor cell lines. The complex exhibits strong cytotoxicity, whereby the leukaemia- and lymphoma-derived cell lines are more sensitive, with IC(50) values comparable to those of the reference anticancer drug cisplatin. In contrast, the solid tumor models are more sensitive to the platinum drug. A comparative assessment of both agents against the human kidney cell line 293T has shown that [Au(II)Hp(−2H).(H(2)O)(2)] is less cytotoxic. The gold complex induces oligonucleosomal DNA fragmentation in tumour cells following 24-hour treatment and hence its cytotoxic effect is at least partly mediated by induction of apoptotic cell death. A prominent intracellular gold accumulation was detected after treating tumor cells with [Au(II)Hp(−2H).(H(2)O)(2)] which shows that its putative pharmacological targets are readily accessible after a short incubation period. Hindawi Publishing Corporation 2008 2008-05-15 /pmc/articles/PMC2386889/ /pubmed/18497870 http://dx.doi.org/10.1155/2008/367471 Text en Copyright © 2008 Georgi Momekov et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Momekov, Georgi
Ferdinandov, Dilyan
Konstantinov, Spiro
Arpadjan, Sonja
Tsekova, Daniela
Gencheva, Galina
Bontchev, Panayot R.
Karaivanova, Margarita
In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis
title In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis
title_full In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis
title_fullStr In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis
title_full_unstemmed In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis
title_short In Vitro Evaluation of a Stable Monomeric Gold(II) Complex with Hematoporphyrin IX: Cytotoxicity against Tumor and Kidney Cells, Cellular Accumulation, and Induction of Apoptosis
title_sort in vitro evaluation of a stable monomeric gold(ii) complex with hematoporphyrin ix: cytotoxicity against tumor and kidney cells, cellular accumulation, and induction of apoptosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386889/
https://www.ncbi.nlm.nih.gov/pubmed/18497870
http://dx.doi.org/10.1155/2008/367471
work_keys_str_mv AT momekovgeorgi invitroevaluationofastablemonomericgoldiicomplexwithhematoporphyrinixcytotoxicityagainsttumorandkidneycellscellularaccumulationandinductionofapoptosis
AT ferdinandovdilyan invitroevaluationofastablemonomericgoldiicomplexwithhematoporphyrinixcytotoxicityagainsttumorandkidneycellscellularaccumulationandinductionofapoptosis
AT konstantinovspiro invitroevaluationofastablemonomericgoldiicomplexwithhematoporphyrinixcytotoxicityagainsttumorandkidneycellscellularaccumulationandinductionofapoptosis
AT arpadjansonja invitroevaluationofastablemonomericgoldiicomplexwithhematoporphyrinixcytotoxicityagainsttumorandkidneycellscellularaccumulationandinductionofapoptosis
AT tsekovadaniela invitroevaluationofastablemonomericgoldiicomplexwithhematoporphyrinixcytotoxicityagainsttumorandkidneycellscellularaccumulationandinductionofapoptosis
AT genchevagalina invitroevaluationofastablemonomericgoldiicomplexwithhematoporphyrinixcytotoxicityagainsttumorandkidneycellscellularaccumulationandinductionofapoptosis
AT bontchevpanayotr invitroevaluationofastablemonomericgoldiicomplexwithhematoporphyrinixcytotoxicityagainsttumorandkidneycellscellularaccumulationandinductionofapoptosis
AT karaivanovamargarita invitroevaluationofastablemonomericgoldiicomplexwithhematoporphyrinixcytotoxicityagainsttumorandkidneycellscellularaccumulationandinductionofapoptosis