Identification of soluble protein fragments by gene fragmentation and genetic selection

We describe a new method, which identifies protein fragments for soluble expression in Escherichia coli from a randomly fragmented gene library. Inhibition of E. coli dihydrofolate reductase (DHFR) by trimethoprim (TMP) prevents growth, but this can be relieved by murine DHFR (mDHFR). Bacterial stra...

Descripción completa

Detalles Bibliográficos
Autores principales: Dyson, Michael R., Perera, Rajika L., Shadbolt, S. Paul, Biderman, Lynn, Bromek, Krystyna, Murzina, Natalia V., McCafferty, John
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396403/
https://www.ncbi.nlm.nih.gov/pubmed/18420658
http://dx.doi.org/10.1093/nar/gkn151

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396403/
https://www.ncbi.nlm.nih.gov/pubmed/18420658
http://dx.doi.org/10.1093/nar/gkn151

Ejemplares similares