Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis

BACKGROUND: One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for structural and functional studies. Here a series of ligation independent clonin...

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Detalles Bibliográficos
Autores principales: Qin, Huajun, Hu, Jian, Hua, Yuanzhi, Challa, Shridhar V, Cross, Timothy A, Gao, Fei P
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396618/
https://www.ncbi.nlm.nih.gov/pubmed/18485215
http://dx.doi.org/10.1186/1472-6750-8-51

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396618/
https://www.ncbi.nlm.nih.gov/pubmed/18485215
http://dx.doi.org/10.1186/1472-6750-8-51

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