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Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis
BACKGROUND: One of the major challenges for membrane protein structural genomics is establishing high-throughput cloning and expression screening methods to obtain enough purified protein in a homogeneous preparation for structural and functional studies. Here a series of ligation independent clonin...
Autores principales: | Qin, Huajun, Hu, Jian, Hua, Yuanzhi, Challa, Shridhar V, Cross, Timothy A, Gao, Fei P |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2396618/ https://www.ncbi.nlm.nih.gov/pubmed/18485215 http://dx.doi.org/10.1186/1472-6750-8-51 |
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