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A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR

BACKGROUND: Based upon defining a common reference point, current real-time quantitative PCR technologies compare relative differences in amplification profile position. As such, absolute quantification requires construction of target-specific standard curves that are highly resource intensive and p...

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Detalles Bibliográficos
Autores principales: Rutledge, Robert G, Stewart, Don
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2397388/
https://www.ncbi.nlm.nih.gov/pubmed/18466619
http://dx.doi.org/10.1186/1472-6750-8-47