Lipid spirals in Bacillus subtilis and their role in cell division
The fluid mosaic model of membrane structure has been revised in recent years as it has become evident that domains of different lipid composition are present in eukaryotic and prokaryotic cells. Using membrane binding fluorescent dyes, we demonstrate the presence of lipid spirals extending along th...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Blackwell Publishing Ltd
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408660/ https://www.ncbi.nlm.nih.gov/pubmed/18430139 http://dx.doi.org/10.1111/j.1365-2958.2008.06236.x |
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author | Barák, Imrich Muchová, Katarína Wilkinson, Anthony J O'Toole, Peter J Pavlendová, Nada |
author_facet | Barák, Imrich Muchová, Katarína Wilkinson, Anthony J O'Toole, Peter J Pavlendová, Nada |
author_sort | Barák, Imrich |
collection | PubMed |
description | The fluid mosaic model of membrane structure has been revised in recent years as it has become evident that domains of different lipid composition are present in eukaryotic and prokaryotic cells. Using membrane binding fluorescent dyes, we demonstrate the presence of lipid spirals extending along the long axis of cells of the rod-shaped bacterium Bacillus subtilis. These spiral structures are absent from cells in which the synthesis of phosphatidylglycerol is disrupted, suggesting an enrichment in anionic phospholipids. Green fluorescent protein fusions of the cell division protein MinD also form spiral structures and these were shown by fluorescence resonance energy transfer to be coincident with the lipid spirals. These data indicate a higher level of membrane lipid organization than previously observed and a primary role for lipid spirals in determining the site of cell division in bacterial cells. |
format | Text |
id | pubmed-2408660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-24086602008-06-09 Lipid spirals in Bacillus subtilis and their role in cell division Barák, Imrich Muchová, Katarína Wilkinson, Anthony J O'Toole, Peter J Pavlendová, Nada Mol Microbiol Research Articles The fluid mosaic model of membrane structure has been revised in recent years as it has become evident that domains of different lipid composition are present in eukaryotic and prokaryotic cells. Using membrane binding fluorescent dyes, we demonstrate the presence of lipid spirals extending along the long axis of cells of the rod-shaped bacterium Bacillus subtilis. These spiral structures are absent from cells in which the synthesis of phosphatidylglycerol is disrupted, suggesting an enrichment in anionic phospholipids. Green fluorescent protein fusions of the cell division protein MinD also form spiral structures and these were shown by fluorescence resonance energy transfer to be coincident with the lipid spirals. These data indicate a higher level of membrane lipid organization than previously observed and a primary role for lipid spirals in determining the site of cell division in bacterial cells. Blackwell Publishing Ltd 2008-06 /pmc/articles/PMC2408660/ /pubmed/18430139 http://dx.doi.org/10.1111/j.1365-2958.2008.06236.x Text en © 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd https://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation. |
spellingShingle | Research Articles Barák, Imrich Muchová, Katarína Wilkinson, Anthony J O'Toole, Peter J Pavlendová, Nada Lipid spirals in Bacillus subtilis and their role in cell division |
title | Lipid spirals in Bacillus subtilis and their role in cell division |
title_full | Lipid spirals in Bacillus subtilis and their role in cell division |
title_fullStr | Lipid spirals in Bacillus subtilis and their role in cell division |
title_full_unstemmed | Lipid spirals in Bacillus subtilis and their role in cell division |
title_short | Lipid spirals in Bacillus subtilis and their role in cell division |
title_sort | lipid spirals in bacillus subtilis and their role in cell division |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408660/ https://www.ncbi.nlm.nih.gov/pubmed/18430139 http://dx.doi.org/10.1111/j.1365-2958.2008.06236.x |
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