Lipid spirals in Bacillus subtilis and their role in cell division

The fluid mosaic model of membrane structure has been revised in recent years as it has become evident that domains of different lipid composition are present in eukaryotic and prokaryotic cells. Using membrane binding fluorescent dyes, we demonstrate the presence of lipid spirals extending along th...

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Autores principales: Barák, Imrich, Muchová, Katarína, Wilkinson, Anthony J, O'Toole, Peter J, Pavlendová, Nada
Formato: Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408660/
https://www.ncbi.nlm.nih.gov/pubmed/18430139
http://dx.doi.org/10.1111/j.1365-2958.2008.06236.x
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author Barák, Imrich
Muchová, Katarína
Wilkinson, Anthony J
O'Toole, Peter J
Pavlendová, Nada
author_facet Barák, Imrich
Muchová, Katarína
Wilkinson, Anthony J
O'Toole, Peter J
Pavlendová, Nada
author_sort Barák, Imrich
collection PubMed
description The fluid mosaic model of membrane structure has been revised in recent years as it has become evident that domains of different lipid composition are present in eukaryotic and prokaryotic cells. Using membrane binding fluorescent dyes, we demonstrate the presence of lipid spirals extending along the long axis of cells of the rod-shaped bacterium Bacillus subtilis. These spiral structures are absent from cells in which the synthesis of phosphatidylglycerol is disrupted, suggesting an enrichment in anionic phospholipids. Green fluorescent protein fusions of the cell division protein MinD also form spiral structures and these were shown by fluorescence resonance energy transfer to be coincident with the lipid spirals. These data indicate a higher level of membrane lipid organization than previously observed and a primary role for lipid spirals in determining the site of cell division in bacterial cells.
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spelling pubmed-24086602008-06-09 Lipid spirals in Bacillus subtilis and their role in cell division Barák, Imrich Muchová, Katarína Wilkinson, Anthony J O'Toole, Peter J Pavlendová, Nada Mol Microbiol Research Articles The fluid mosaic model of membrane structure has been revised in recent years as it has become evident that domains of different lipid composition are present in eukaryotic and prokaryotic cells. Using membrane binding fluorescent dyes, we demonstrate the presence of lipid spirals extending along the long axis of cells of the rod-shaped bacterium Bacillus subtilis. These spiral structures are absent from cells in which the synthesis of phosphatidylglycerol is disrupted, suggesting an enrichment in anionic phospholipids. Green fluorescent protein fusions of the cell division protein MinD also form spiral structures and these were shown by fluorescence resonance energy transfer to be coincident with the lipid spirals. These data indicate a higher level of membrane lipid organization than previously observed and a primary role for lipid spirals in determining the site of cell division in bacterial cells. Blackwell Publishing Ltd 2008-06 /pmc/articles/PMC2408660/ /pubmed/18430139 http://dx.doi.org/10.1111/j.1365-2958.2008.06236.x Text en © 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd https://creativecommons.org/licenses/by/2.5/ Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.
spellingShingle Research Articles
Barák, Imrich
Muchová, Katarína
Wilkinson, Anthony J
O'Toole, Peter J
Pavlendová, Nada
Lipid spirals in Bacillus subtilis and their role in cell division
title Lipid spirals in Bacillus subtilis and their role in cell division
title_full Lipid spirals in Bacillus subtilis and their role in cell division
title_fullStr Lipid spirals in Bacillus subtilis and their role in cell division
title_full_unstemmed Lipid spirals in Bacillus subtilis and their role in cell division
title_short Lipid spirals in Bacillus subtilis and their role in cell division
title_sort lipid spirals in bacillus subtilis and their role in cell division
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2408660/
https://www.ncbi.nlm.nih.gov/pubmed/18430139
http://dx.doi.org/10.1111/j.1365-2958.2008.06236.x
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