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Sequence polymorphism can produce serious artefacts in real-time PCR assays: hard lessons from Pacific oysters
BACKGROUND: Since it was first described in the mid-1990s, quantitative real time PCR (Q-PCR) has been widely used in many fields of biomedical research and molecular diagnostics. This method is routinely used to validate whole transcriptome analyses such as DNA microarrays, suppressive subtractive...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
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BioMed Central
2008
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2424065/ https://www.ncbi.nlm.nih.gov/pubmed/18492266 http://dx.doi.org/10.1186/1471-2164-9-234 |