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Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo
The zebrafish u-boot (ubo) gene encodes the transcription factor Prdm1, which is essential for the specification of the primary slow-twitch muscle fibres that derive from adaxial cells. Here, we show that Prdm1 functions by acting as a transcriptional repressor and that slow-twitch-specific muscle g...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2424280/ https://www.ncbi.nlm.nih.gov/pubmed/18535625 http://dx.doi.org/10.1038/embor.2008.73 |
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author | von Hofsten, Jonas Elworthy, Stone Gilchrist, Michael J Smith, James C Wardle, Fiona C Ingham, Philip W |
author_facet | von Hofsten, Jonas Elworthy, Stone Gilchrist, Michael J Smith, James C Wardle, Fiona C Ingham, Philip W |
author_sort | von Hofsten, Jonas |
collection | PubMed |
description | The zebrafish u-boot (ubo) gene encodes the transcription factor Prdm1, which is essential for the specification of the primary slow-twitch muscle fibres that derive from adaxial cells. Here, we show that Prdm1 functions by acting as a transcriptional repressor and that slow-twitch-specific muscle gene expression is activated by Prdm1-mediated repression of the transcriptional repressor Sox6. Genes encoding fast-specific isoforms of sarcomeric proteins are ectopically expressed in the adaxial cells of ubo(tp39) mutant embryos. By using chromatin immunoprecipitation, we show that these are direct targets of Prdm1. Thus, Prdm1 promotes slow-twitch fibre differentiation by acting as a global repressor of fast-fibre-specific genes, as well as by abrogating the repression of slow-fibre-specific genes. |
format | Text |
id | pubmed-2424280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-24242802008-07-22 Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo von Hofsten, Jonas Elworthy, Stone Gilchrist, Michael J Smith, James C Wardle, Fiona C Ingham, Philip W EMBO Rep Scientific Report The zebrafish u-boot (ubo) gene encodes the transcription factor Prdm1, which is essential for the specification of the primary slow-twitch muscle fibres that derive from adaxial cells. Here, we show that Prdm1 functions by acting as a transcriptional repressor and that slow-twitch-specific muscle gene expression is activated by Prdm1-mediated repression of the transcriptional repressor Sox6. Genes encoding fast-specific isoforms of sarcomeric proteins are ectopically expressed in the adaxial cells of ubo(tp39) mutant embryos. By using chromatin immunoprecipitation, we show that these are direct targets of Prdm1. Thus, Prdm1 promotes slow-twitch fibre differentiation by acting as a global repressor of fast-fibre-specific genes, as well as by abrogating the repression of slow-fibre-specific genes. Nature Publishing Group 2008-07 2008-06-06 /pmc/articles/PMC2424280/ /pubmed/18535625 http://dx.doi.org/10.1038/embor.2008.73 Text en Copyright © 2008, European Molecular Biology Organization http://creativecommons.org/licenses/by-nc-nd/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission. |
spellingShingle | Scientific Report von Hofsten, Jonas Elworthy, Stone Gilchrist, Michael J Smith, James C Wardle, Fiona C Ingham, Philip W Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo |
title | Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo |
title_full | Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo |
title_fullStr | Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo |
title_full_unstemmed | Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo |
title_short | Prdm1- and Sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo |
title_sort | prdm1- and sox6-mediated transcriptional repression specifies muscle fibre type in the zebrafish embryo |
topic | Scientific Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2424280/ https://www.ncbi.nlm.nih.gov/pubmed/18535625 http://dx.doi.org/10.1038/embor.2008.73 |
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