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Chemical mapping of cytosines enzymatically flipped out of the DNA helix
Haloacetaldehydes can be employed for probing unpaired DNA structures involving cytosine and adenine residues. Using an enzyme that was structurally proven to flip its target cytosine out of the DNA helix, the HhaI DNA methyltransferase (M.HhaI), we demonstrate the suitability of the chloroacetaldeh...
| Hlavní autoři: | , , , |
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| Médium: | Text |
| Jazyk: | English |
| Vydáno: |
Oxford University Press
2008
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| On-line přístup: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2425465/ https://www.ncbi.nlm.nih.gov/pubmed/18450817 http://dx.doi.org/10.1093/nar/gkn200 |