Chemical mapping of cytosines enzymatically flipped out of the DNA helix

Haloacetaldehydes can be employed for probing unpaired DNA structures involving cytosine and adenine residues. Using an enzyme that was structurally proven to flip its target cytosine out of the DNA helix, the HhaI DNA methyltransferase (M.HhaI), we demonstrate the suitability of the chloroacetaldeh...

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Bibliographic Details
Main Authors: Daujotytė, Dalia, Liutkevičiūtė, Zita, Tamulaitis, Gintautas, Klimašauskas, Saulius
Format: Text
Language:English
Published: Oxford University Press 2008
Subjects:
Methods Online
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2425465/
https://www.ncbi.nlm.nih.gov/pubmed/18450817
http://dx.doi.org/10.1093/nar/gkn200

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2425465/
https://www.ncbi.nlm.nih.gov/pubmed/18450817
http://dx.doi.org/10.1093/nar/gkn200

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