Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement (‘gating’ current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a gene...

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Detalles Bibliográficos
Autores principales: Lundby, Alicia, Mutoh, Hiroki, Dimitrov, Dimitar, Akemann, Walther, Knöpfel, Thomas
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2429971/
https://www.ncbi.nlm.nih.gov/pubmed/18575613
http://dx.doi.org/10.1371/journal.pone.0002514
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author Lundby, Alicia
Mutoh, Hiroki
Dimitrov, Dimitar
Akemann, Walther
Knöpfel, Thomas
author_facet Lundby, Alicia
Mutoh, Hiroki
Dimitrov, Dimitar
Akemann, Walther
Knöpfel, Thomas
author_sort Lundby, Alicia
collection PubMed
description Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement (‘gating’ current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.
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spelling pubmed-24299712008-06-25 Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements Lundby, Alicia Mutoh, Hiroki Dimitrov, Dimitar Akemann, Walther Knöpfel, Thomas PLoS One Research Article Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement (‘gating’ current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs. Public Library of Science 2008-06-25 /pmc/articles/PMC2429971/ /pubmed/18575613 http://dx.doi.org/10.1371/journal.pone.0002514 Text en Lundby et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lundby, Alicia
Mutoh, Hiroki
Dimitrov, Dimitar
Akemann, Walther
Knöpfel, Thomas
Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements
title Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements
title_full Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements
title_fullStr Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements
title_full_unstemmed Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements
title_short Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements
title_sort engineering of a genetically encodable fluorescent voltage sensor exploiting fast ci-vsp voltage-sensing movements
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2429971/
https://www.ncbi.nlm.nih.gov/pubmed/18575613
http://dx.doi.org/10.1371/journal.pone.0002514
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