Marking Embryonic Stem Cells with a 2A Self-Cleaving Peptide: A NKX2-5 Emerald GFP BAC Reporter

BACKGROUND: Fluorescent reporters are useful for assaying gene expression in living cells and for identifying and isolating pure cell populations from heterogeneous cultures, including embryonic stem (ES) cells. Multiple fluorophores and genetic selection markers exist; however, a system for creatin...

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Detalles Bibliográficos
Autores principales: Hsiao, Edward C., Yoshinaga, Yuko, Nguyen, Trieu D., Musone, Stacy L., Kim, Judy E., Swinton, Paul, Espineda, Isidro, Manalac, Carlota, deJong, Pieter J., Conklin, Bruce R.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2430532/
https://www.ncbi.nlm.nih.gov/pubmed/18596956
http://dx.doi.org/10.1371/journal.pone.0002532

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2430532/
https://www.ncbi.nlm.nih.gov/pubmed/18596956
http://dx.doi.org/10.1371/journal.pone.0002532

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