Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA

BACKGROUND: In a traditional electrophoresis mobility shift assay (EMSA) a (32)P-labeled double-stranded DNA oligonucleotide or a restriction fragment bound to a protein is separated from the unbound DNA by polyacrylamide gel electrophoresis (PAGE) in nondenaturing conditions. An extension of this m...

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Detalles Bibliográficos
Autores principales: Kaer, Kristel, Mätlik, Kert, Metsis, Madis, Speek, Mart
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435560/
https://www.ncbi.nlm.nih.gov/pubmed/18533036
http://dx.doi.org/10.1186/1471-2164-9-272

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435560/
https://www.ncbi.nlm.nih.gov/pubmed/18533036
http://dx.doi.org/10.1186/1471-2164-9-272

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