Cargando…
Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA
BACKGROUND: In a traditional electrophoresis mobility shift assay (EMSA) a (32)P-labeled double-stranded DNA oligonucleotide or a restriction fragment bound to a protein is separated from the unbound DNA by polyacrylamide gel electrophoresis (PAGE) in nondenaturing conditions. An extension of this m...
| Autores principales: | , , , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2008
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2435560/ https://www.ncbi.nlm.nih.gov/pubmed/18533036 http://dx.doi.org/10.1186/1471-2164-9-272 |