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Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system

Rapid nerve impulse conduction in myelinated axons requires the concentration of voltage-gated sodium channels at nodes of Ranvier. Myelin-forming oligodendrocytes in the central nervous system (CNS) induce the clustering of sodium channels into nodal complexes flanked by paranodal axoglial junction...

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Autores principales: Zonta, Barbara, Tait, Steven, Melrose, Shona, Anderson, Heather, Harroch, Sheila, Higginson, Jennifer, Sherman, Diane L., Brophy, Peter J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442198/
https://www.ncbi.nlm.nih.gov/pubmed/18573915
http://dx.doi.org/10.1083/jcb.200712154
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author Zonta, Barbara
Tait, Steven
Melrose, Shona
Anderson, Heather
Harroch, Sheila
Higginson, Jennifer
Sherman, Diane L.
Brophy, Peter J.
author_facet Zonta, Barbara
Tait, Steven
Melrose, Shona
Anderson, Heather
Harroch, Sheila
Higginson, Jennifer
Sherman, Diane L.
Brophy, Peter J.
author_sort Zonta, Barbara
collection PubMed
description Rapid nerve impulse conduction in myelinated axons requires the concentration of voltage-gated sodium channels at nodes of Ranvier. Myelin-forming oligodendrocytes in the central nervous system (CNS) induce the clustering of sodium channels into nodal complexes flanked by paranodal axoglial junctions. However, the molecular mechanisms for nodal complex assembly in the CNS are unknown. Two isoforms of Neurofascin, neuronal Nfasc186 and glial Nfasc155, are components of the nodal and paranodal complexes, respectively. Neurofascin-null mice have disrupted nodal and paranodal complexes. We show that transgenic Nfasc186 can rescue the nodal complex when expressed in Nfasc(−/−) mice in the absence of the Nfasc155–Caspr–Contactin adhesion complex. Reconstitution of the axoglial adhesion complex by expressing transgenic Nfasc155 in oligodendrocytes also rescues the nodal complex independently of Nfasc186. Furthermore, the Nfasc155 adhesion complex has an additional function in promoting the migration of myelinating processes along CNS axons. We propose that glial and neuronal Neurofascins have distinct functions in the assembly of the CNS node of Ranvier.
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spelling pubmed-24421982008-12-30 Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system Zonta, Barbara Tait, Steven Melrose, Shona Anderson, Heather Harroch, Sheila Higginson, Jennifer Sherman, Diane L. Brophy, Peter J. J Cell Biol Research Articles Rapid nerve impulse conduction in myelinated axons requires the concentration of voltage-gated sodium channels at nodes of Ranvier. Myelin-forming oligodendrocytes in the central nervous system (CNS) induce the clustering of sodium channels into nodal complexes flanked by paranodal axoglial junctions. However, the molecular mechanisms for nodal complex assembly in the CNS are unknown. Two isoforms of Neurofascin, neuronal Nfasc186 and glial Nfasc155, are components of the nodal and paranodal complexes, respectively. Neurofascin-null mice have disrupted nodal and paranodal complexes. We show that transgenic Nfasc186 can rescue the nodal complex when expressed in Nfasc(−/−) mice in the absence of the Nfasc155–Caspr–Contactin adhesion complex. Reconstitution of the axoglial adhesion complex by expressing transgenic Nfasc155 in oligodendrocytes also rescues the nodal complex independently of Nfasc186. Furthermore, the Nfasc155 adhesion complex has an additional function in promoting the migration of myelinating processes along CNS axons. We propose that glial and neuronal Neurofascins have distinct functions in the assembly of the CNS node of Ranvier. The Rockefeller University Press 2008-06-30 /pmc/articles/PMC2442198/ /pubmed/18573915 http://dx.doi.org/10.1083/jcb.200712154 Text en © 2008 Zonta et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Zonta, Barbara
Tait, Steven
Melrose, Shona
Anderson, Heather
Harroch, Sheila
Higginson, Jennifer
Sherman, Diane L.
Brophy, Peter J.
Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system
title Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system
title_full Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system
title_fullStr Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system
title_full_unstemmed Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system
title_short Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system
title_sort glial and neuronal isoforms of neurofascin have distinct roles in the assembly of nodes of ranvier in the central nervous system
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442198/
https://www.ncbi.nlm.nih.gov/pubmed/18573915
http://dx.doi.org/10.1083/jcb.200712154
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