Cargando…
Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system
Rapid nerve impulse conduction in myelinated axons requires the concentration of voltage-gated sodium channels at nodes of Ranvier. Myelin-forming oligodendrocytes in the central nervous system (CNS) induce the clustering of sodium channels into nodal complexes flanked by paranodal axoglial junction...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442198/ https://www.ncbi.nlm.nih.gov/pubmed/18573915 http://dx.doi.org/10.1083/jcb.200712154 |
_version_ | 1782156690757517312 |
---|---|
author | Zonta, Barbara Tait, Steven Melrose, Shona Anderson, Heather Harroch, Sheila Higginson, Jennifer Sherman, Diane L. Brophy, Peter J. |
author_facet | Zonta, Barbara Tait, Steven Melrose, Shona Anderson, Heather Harroch, Sheila Higginson, Jennifer Sherman, Diane L. Brophy, Peter J. |
author_sort | Zonta, Barbara |
collection | PubMed |
description | Rapid nerve impulse conduction in myelinated axons requires the concentration of voltage-gated sodium channels at nodes of Ranvier. Myelin-forming oligodendrocytes in the central nervous system (CNS) induce the clustering of sodium channels into nodal complexes flanked by paranodal axoglial junctions. However, the molecular mechanisms for nodal complex assembly in the CNS are unknown. Two isoforms of Neurofascin, neuronal Nfasc186 and glial Nfasc155, are components of the nodal and paranodal complexes, respectively. Neurofascin-null mice have disrupted nodal and paranodal complexes. We show that transgenic Nfasc186 can rescue the nodal complex when expressed in Nfasc(−/−) mice in the absence of the Nfasc155–Caspr–Contactin adhesion complex. Reconstitution of the axoglial adhesion complex by expressing transgenic Nfasc155 in oligodendrocytes also rescues the nodal complex independently of Nfasc186. Furthermore, the Nfasc155 adhesion complex has an additional function in promoting the migration of myelinating processes along CNS axons. We propose that glial and neuronal Neurofascins have distinct functions in the assembly of the CNS node of Ranvier. |
format | Text |
id | pubmed-2442198 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-24421982008-12-30 Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system Zonta, Barbara Tait, Steven Melrose, Shona Anderson, Heather Harroch, Sheila Higginson, Jennifer Sherman, Diane L. Brophy, Peter J. J Cell Biol Research Articles Rapid nerve impulse conduction in myelinated axons requires the concentration of voltage-gated sodium channels at nodes of Ranvier. Myelin-forming oligodendrocytes in the central nervous system (CNS) induce the clustering of sodium channels into nodal complexes flanked by paranodal axoglial junctions. However, the molecular mechanisms for nodal complex assembly in the CNS are unknown. Two isoforms of Neurofascin, neuronal Nfasc186 and glial Nfasc155, are components of the nodal and paranodal complexes, respectively. Neurofascin-null mice have disrupted nodal and paranodal complexes. We show that transgenic Nfasc186 can rescue the nodal complex when expressed in Nfasc(−/−) mice in the absence of the Nfasc155–Caspr–Contactin adhesion complex. Reconstitution of the axoglial adhesion complex by expressing transgenic Nfasc155 in oligodendrocytes also rescues the nodal complex independently of Nfasc186. Furthermore, the Nfasc155 adhesion complex has an additional function in promoting the migration of myelinating processes along CNS axons. We propose that glial and neuronal Neurofascins have distinct functions in the assembly of the CNS node of Ranvier. The Rockefeller University Press 2008-06-30 /pmc/articles/PMC2442198/ /pubmed/18573915 http://dx.doi.org/10.1083/jcb.200712154 Text en © 2008 Zonta et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Zonta, Barbara Tait, Steven Melrose, Shona Anderson, Heather Harroch, Sheila Higginson, Jennifer Sherman, Diane L. Brophy, Peter J. Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system |
title | Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system |
title_full | Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system |
title_fullStr | Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system |
title_full_unstemmed | Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system |
title_short | Glial and neuronal isoforms of Neurofascin have distinct roles in the assembly of nodes of Ranvier in the central nervous system |
title_sort | glial and neuronal isoforms of neurofascin have distinct roles in the assembly of nodes of ranvier in the central nervous system |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2442198/ https://www.ncbi.nlm.nih.gov/pubmed/18573915 http://dx.doi.org/10.1083/jcb.200712154 |
work_keys_str_mv | AT zontabarbara glialandneuronalisoformsofneurofascinhavedistinctrolesintheassemblyofnodesofranvierinthecentralnervoussystem AT taitsteven glialandneuronalisoformsofneurofascinhavedistinctrolesintheassemblyofnodesofranvierinthecentralnervoussystem AT melroseshona glialandneuronalisoformsofneurofascinhavedistinctrolesintheassemblyofnodesofranvierinthecentralnervoussystem AT andersonheather glialandneuronalisoformsofneurofascinhavedistinctrolesintheassemblyofnodesofranvierinthecentralnervoussystem AT harrochsheila glialandneuronalisoformsofneurofascinhavedistinctrolesintheassemblyofnodesofranvierinthecentralnervoussystem AT higginsonjennifer glialandneuronalisoformsofneurofascinhavedistinctrolesintheassemblyofnodesofranvierinthecentralnervoussystem AT shermandianel glialandneuronalisoformsofneurofascinhavedistinctrolesintheassemblyofnodesofranvierinthecentralnervoussystem AT brophypeterj glialandneuronalisoformsofneurofascinhavedistinctrolesintheassemblyofnodesofranvierinthecentralnervoussystem |