Template-directed addition of nucleosides to DNA by the BfiI restriction enzyme

Restriction endonucleases catalyse DNA cleavage at specific sites. The BfiI endonuclease cuts DNA to give staggered ends with 1-nt 3′-extensions. We show here that BfiI can also fill in the staggered ends: while cleaving DNA, it can add a 2′-deoxynucleoside to the reaction product to yield directly...

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Detalles Bibliográficos
Autores principales: Sasnauskas, Giedrius, Connolly, Bernard A., Halford, Stephen E., Siksnys, Virginijus
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475615/
https://www.ncbi.nlm.nih.gov/pubmed/18515343
http://dx.doi.org/10.1093/nar/gkn343

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475615/
https://www.ncbi.nlm.nih.gov/pubmed/18515343
http://dx.doi.org/10.1093/nar/gkn343

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