Efficient cytosolic delivery of molecular beacon conjugates and flow cytometric analysis of target RNA

Fluorescent microscopy experiments show that when 2′-O-methyl-modified molecular beacons (MBs) are introduced into NIH/3T3 cells, they elicit a nonspecific signal in the nucleus. This false-positive signal can be avoided by conjugating MBs to macromolecules (e.g. NeutrAvidin) that prevent nuclear se...

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Detalles Bibliográficos
Autores principales: Chen, Antony K., Behlke, Mark A., Tsourkas, Andrew
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475621/
https://www.ncbi.nlm.nih.gov/pubmed/18503086
http://dx.doi.org/10.1093/nar/gkn331

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475621/
https://www.ncbi.nlm.nih.gov/pubmed/18503086
http://dx.doi.org/10.1093/nar/gkn331

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