Cargando…
Efficient cytosolic delivery of molecular beacon conjugates and flow cytometric analysis of target RNA
Fluorescent microscopy experiments show that when 2′-O-methyl-modified molecular beacons (MBs) are introduced into NIH/3T3 cells, they elicit a nonspecific signal in the nucleus. This false-positive signal can be avoided by conjugating MBs to macromolecules (e.g. NeutrAvidin) that prevent nuclear se...
| Autores principales: | , , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2008
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475621/ https://www.ncbi.nlm.nih.gov/pubmed/18503086 http://dx.doi.org/10.1093/nar/gkn331 |