Single-molecule imaging of full protein synthesis by immobilized ribosomes

How folding of proteins is coupled to their synthesis remains poorly understood. Here, we apply single-molecule fluorescence imaging to full protein synthesis in vitro. Ribosomes were specifically immobilized onto glass surfaces and synthesis of green fluorescent protein (GFP) was achieved using mod...

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Detalles Bibliográficos
Autores principales: Uemura, Sotaro, Iizuka, Ryo, Ueno, Taro, Shimizu, Yoshihiro, Taguchi, Hideki, Ueda, Takuya, Puglisi, Joseph D., Funatsu, Takashi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475623/
https://www.ncbi.nlm.nih.gov/pubmed/18511463
http://dx.doi.org/10.1093/nar/gkn338

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475623/
https://www.ncbi.nlm.nih.gov/pubmed/18511463
http://dx.doi.org/10.1093/nar/gkn338

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