Inversing the natural hydrogen bonding rule to selectively amplify GC-rich ADAR-edited RNAs

DNA complementarity is expressed by way of three hydrogen bonds for a G:C base pair and two for A:T. As a result, careful control of the denaturation temperature of PCR allows selective amplification of AT-rich alleles. Yet for the same reason, the converse is not possible, selective amplification o...

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Detalles Bibliográficos
Autores principales: Suspène, Rodolphe, Renard, Myrtille, Henry, Michel, Guétard, Denise, Puyraimond-Zemmour, David, Billecocq, Agnès, Bouloy, Michèle, Tangy, Frederic, Vartanian, Jean-Pierre, Wain-Hobson, Simon
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475633/
https://www.ncbi.nlm.nih.gov/pubmed/18515351
http://dx.doi.org/10.1093/nar/gkn295

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475633/
https://www.ncbi.nlm.nih.gov/pubmed/18515351
http://dx.doi.org/10.1093/nar/gkn295

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