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Inversing the natural hydrogen bonding rule to selectively amplify GC-rich ADAR-edited RNAs
DNA complementarity is expressed by way of three hydrogen bonds for a G:C base pair and two for A:T. As a result, careful control of the denaturation temperature of PCR allows selective amplification of AT-rich alleles. Yet for the same reason, the converse is not possible, selective amplification o...
| Autores principales: | , , , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475633/ https://www.ncbi.nlm.nih.gov/pubmed/18515351 http://dx.doi.org/10.1093/nar/gkn295 |