Minimally invasive determination of mRNA concentration in single living bacteria

Fluorescence correlation spectroscopy (FCS) has permitted the characterization of high concentrations of noncoding RNAs in a single living bacterium. Here, we extend the use of FCS to low concentrations of coding RNAs in single living cells. We genetically fuse a red fluorescent protein (RFP) gene a...

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Detalles Bibliográficos
Autores principales: Guet, Călin C., Bruneaux, Luke, Min, Taejin L., Siegal-Gaskins, Dan, Figueroa, Israel, Emonet, Thierry, Cluzel, Philippe
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475643/
https://www.ncbi.nlm.nih.gov/pubmed/18515347
http://dx.doi.org/10.1093/nar/gkn329

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2475643/
https://www.ncbi.nlm.nih.gov/pubmed/18515347
http://dx.doi.org/10.1093/nar/gkn329

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