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C-Peptide Prevents Hippocampal Apoptosis in Type 1 Diabetes

To explore mechanisms underlying central nervous system (CNS) complications in diabetes, we examined hippocampal neuronal apoptosis and loss, and the effect of C-peptide replacement in type 1 diabetic BB/W rats. Apoptosis was demonstrated after 8 months of diabetes, by DNA fragmentation, increased n...

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Detalles Bibliográficos
Autores principales: Li, Zhen-guo, Zhang, Weixian, Sima, Anders A. F.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2002
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2478591/
https://www.ncbi.nlm.nih.gov/pubmed/12546277
http://dx.doi.org/10.1080/15604280214936
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author Li, Zhen-guo
Zhang, Weixian
Sima, Anders A. F.
author_facet Li, Zhen-guo
Zhang, Weixian
Sima, Anders A. F.
author_sort Li, Zhen-guo
collection PubMed
description To explore mechanisms underlying central nervous system (CNS) complications in diabetes, we examined hippocampal neuronal apoptosis and loss, and the effect of C-peptide replacement in type 1 diabetic BB/W rats. Apoptosis was demonstrated after 8 months of diabetes, by DNA fragmentation, increased number of apoptotic cells, and an elevated ratio of Bax/Bcl-x(L), accompanied by reduced neuronal density in the hippocampus. No apoptotic activity was detected and neuronal density was unchanged in 2-month diabetic hippocampus, whereas insulin-like growth factor (IGF) activities were impaired. In type 1 diabetic BB/W rats replaced with C-peptide, no TdT-mediated dUTP nick-end labeling (TUNEL)- positive cells were shown and DNA laddering was not evident in hippocampus at either 2 or 8 months. C-peptide administration prevented the preceding perturbation of IGF expression and reduced the elevated ratio of Bax/Bcl-x(L). Our data suggest that type 1 diabetes causes a duration-dependent programmed cell death of the hippocampus, which is partially prevented by C-peptide.
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spelling pubmed-24785912008-08-18 C-Peptide Prevents Hippocampal Apoptosis in Type 1 Diabetes Li, Zhen-guo Zhang, Weixian Sima, Anders A. F. Int J Exp Diabetes Res Research Article To explore mechanisms underlying central nervous system (CNS) complications in diabetes, we examined hippocampal neuronal apoptosis and loss, and the effect of C-peptide replacement in type 1 diabetic BB/W rats. Apoptosis was demonstrated after 8 months of diabetes, by DNA fragmentation, increased number of apoptotic cells, and an elevated ratio of Bax/Bcl-x(L), accompanied by reduced neuronal density in the hippocampus. No apoptotic activity was detected and neuronal density was unchanged in 2-month diabetic hippocampus, whereas insulin-like growth factor (IGF) activities were impaired. In type 1 diabetic BB/W rats replaced with C-peptide, no TdT-mediated dUTP nick-end labeling (TUNEL)- positive cells were shown and DNA laddering was not evident in hippocampus at either 2 or 8 months. C-peptide administration prevented the preceding perturbation of IGF expression and reduced the elevated ratio of Bax/Bcl-x(L). Our data suggest that type 1 diabetes causes a duration-dependent programmed cell death of the hippocampus, which is partially prevented by C-peptide. Hindawi Publishing Corporation 2002 /pmc/articles/PMC2478591/ /pubmed/12546277 http://dx.doi.org/10.1080/15604280214936 Text en Copyright © 2002 Hindawi Publishing Corporation. http://creativecommons.org/licenses/by/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Zhen-guo
Zhang, Weixian
Sima, Anders A. F.
C-Peptide Prevents Hippocampal Apoptosis in Type 1 Diabetes
title C-Peptide Prevents Hippocampal Apoptosis in Type 1 Diabetes
title_full C-Peptide Prevents Hippocampal Apoptosis in Type 1 Diabetes
title_fullStr C-Peptide Prevents Hippocampal Apoptosis in Type 1 Diabetes
title_full_unstemmed C-Peptide Prevents Hippocampal Apoptosis in Type 1 Diabetes
title_short C-Peptide Prevents Hippocampal Apoptosis in Type 1 Diabetes
title_sort c-peptide prevents hippocampal apoptosis in type 1 diabetes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2478591/
https://www.ncbi.nlm.nih.gov/pubmed/12546277
http://dx.doi.org/10.1080/15604280214936
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