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Purification of human respiratory syncytial virus by ultracentrifugation in iodixanol density gradient
Ultracentrifugation in sucrose density gradient remains the most commonly used technique for hRSV purification. However, the high viscosity and hyper-osmotic property of sucrose can cause damage to the extremely labile virus leading to loss of infectivity. To overcome these limitations, an alternati...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Elsevier/North-Holland Biomedical Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2486346/ https://www.ncbi.nlm.nih.gov/pubmed/18029032 http://dx.doi.org/10.1016/j.jviromet.2007.09.013 |