Primase-based whole genome amplification

In vitro DNA amplification methods, such as polymerase chain reaction (PCR), rely on synthetic oligonucleotide primers for initiation of the reaction. In vivo, primers are synthesized on-template by DNA primase. The bacteriophage T7 gene 4 protein (gp4) has both primase and helicase activities. In t...

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Detalles Bibliográficos
Autores principales: Li, Ying, Kim, Hyun-Jin, Zheng, Chunyang, Chow, Wing Huen A., Lim, Jeonghwa, Keenan, Brendan, Pan, Xiaojing, Lemieux, Bertrand, Kong, Huimin
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2490742/
https://www.ncbi.nlm.nih.gov/pubmed/18559358
http://dx.doi.org/10.1093/nar/gkn377

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2490742/
https://www.ncbi.nlm.nih.gov/pubmed/18559358
http://dx.doi.org/10.1093/nar/gkn377

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