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Rapid and efficient construction of markerless deletions in the Escherichia coli genome
We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504295/ https://www.ncbi.nlm.nih.gov/pubmed/18567910 http://dx.doi.org/10.1093/nar/gkn359 |