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Rapid and efficient construction of markerless deletions in the Escherichia coli genome

We have developed an improved and rapid genomic engineering procedure for the construction of custom-designed microorganisms. This method, which can be performed in 2 days, permits restructuring of the Escherichia coli genome via markerless deletion of selected genomic regions. The deletion process...

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Detalles Bibliográficos
Autores principales:	Yu, Byung Jo, Kang, Kui Hyeon, Lee, Jun Hyoung, Sung, Bong Hyun, Kim, Mi Sun, Kim, Sun Chang
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2008
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504295/ https://www.ncbi.nlm.nih.gov/pubmed/18567910 http://dx.doi.org/10.1093/nar/gkn359

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