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Multiple segmental and selective isotope labeling of large RNA for NMR structural studies

Multiple segmental and selective isotope labeling of RNA with three segments has been demonstrated by introducing an RNA segment, selectively labeled with (13)C(9)/(15)N(2)/(2)H((1′, 3′, 4′, 5′, 5′′))-labeled uridine residues, into the central position of the 20 kDa ε-RNA of Duck Hepatitis B Virus....

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Detalles Bibliográficos
Autores principales: Nelissen, Frank H. T., van Gammeren, Adriaan J., Tessari, Marco, Girard, Frederic C., Heus, Hans A., Wijmenga, Sybren S.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504312/
https://www.ncbi.nlm.nih.gov/pubmed/18583361
http://dx.doi.org/10.1093/nar/gkn397