Cargando…
Multiple segmental and selective isotope labeling of large RNA for NMR structural studies
Multiple segmental and selective isotope labeling of RNA with three segments has been demonstrated by introducing an RNA segment, selectively labeled with (13)C(9)/(15)N(2)/(2)H((1′, 3′, 4′, 5′, 5′′))-labeled uridine residues, into the central position of the 20 kDa ε-RNA of Duck Hepatitis B Virus....
Autores principales: | Nelissen, Frank H. T., van Gammeren, Adriaan J., Tessari, Marco, Girard, Frederic C., Heus, Hans A., Wijmenga, Sybren S. |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504312/ https://www.ncbi.nlm.nih.gov/pubmed/18583361 http://dx.doi.org/10.1093/nar/gkn397 |
Ejemplares similares
-
Preparation of selective and segmentally labeled single-stranded DNA for NMR by self-primed PCR and asymmetrical endonuclease double digestion
por: Nelissen, Frank H. T., et al.
Publicado: (2009) -
Fast production of homogeneous recombinant RNA—towards large-scale production of RNA
por: Nelissen, Frank H.T., et al.
Publicado: (2012) -
Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations
por: Longhini, Andrew P., et al.
Publicado: (2016) -
A fast, efficient and sequence-independent method for flexible multiple segmental isotope labeling of RNA using ribozyme and RNase H cleavage
por: Duss, Olivier, et al.
Publicado: (2010) -
Short, synthetic and selectively (13)C-labeled RNA sequences for the NMR structure determination of protein–RNA complexes
por: Wenter, Philipp, et al.
Publicado: (2006)