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Development of a broad-host-range sacB-based vector for unmarked allelic exchange
BACKGROUND: Although genome sequences are available for an ever-increasing number of bacterial species, the availability of facile genetic tools for physiological analysis have generally lagged substantially behind traditional genetic models. RESULTS: Here I describe the development of an improved,...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2008
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2518277/ https://www.ncbi.nlm.nih.gov/pubmed/18710539 http://dx.doi.org/10.1186/1756-0500-1-1 |