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Repertoire-based selection into the marginal zone compartment during B cell development
Marginal zone (MZ) B cells resemble fetally derived B1 B cells in their innate-like rapid responses to bacterial pathogens, but the basis for this is unknown. We report that the MZ is enriched in “fetal-type” B cell receptors lacking N regions (N(−)). Mixed bone marrow (BM) chimeras, made with adult...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526199/ https://www.ncbi.nlm.nih.gov/pubmed/18710933 http://dx.doi.org/10.1084/jem.20080559 |
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author | Carey, John B. Moffatt-Blue, Chantelle S. Watson, Lisa C. Gavin, Amanda L. Feeney, Ann J. |
author_facet | Carey, John B. Moffatt-Blue, Chantelle S. Watson, Lisa C. Gavin, Amanda L. Feeney, Ann J. |
author_sort | Carey, John B. |
collection | PubMed |
description | Marginal zone (MZ) B cells resemble fetally derived B1 B cells in their innate-like rapid responses to bacterial pathogens, but the basis for this is unknown. We report that the MZ is enriched in “fetal-type” B cell receptors lacking N regions (N(−)). Mixed bone marrow (BM) chimeras, made with adult terminal deoxynucleotidyl transferase (TdT)(+/+) and TdT(−/−) donor cells, demonstrate preferential repertoire-based selection of N(−) B cells into the MZ. Reconstitution of irradiated mice with adult TdT(+/+) BM reveals that the MZ can replenish N(−) B cells in adult life via repertoire-based selection and suggest the possibility of a TdT-deficient precursor population in the adult BM. The mixed chimera data also suggest repertoire-based bifurcations into distinct BM and splenic maturation pathways, with mature “recirculating” BM B cells showing a very strong preference for N(+) complementarity-determining region (CDR) 3 compared with follicular B cells. Because the T1 and MZ compartments are both the most enriched for N(−) H-CDR3, we propose a novel direct T1→MZ pathway and identify a potential T1–MZ precursor intermediate. We demonstrate progressive but discontinuous repertoire-based selection throughout B cell development supporting multiple branchpoints and pathways in B cell development. Multiple differentiation routes leading to MZ development may contribute to the reported functional heterogeneity of the MZ compartment. |
format | Text |
id | pubmed-2526199 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25261992009-03-01 Repertoire-based selection into the marginal zone compartment during B cell development Carey, John B. Moffatt-Blue, Chantelle S. Watson, Lisa C. Gavin, Amanda L. Feeney, Ann J. J Exp Med Articles Marginal zone (MZ) B cells resemble fetally derived B1 B cells in their innate-like rapid responses to bacterial pathogens, but the basis for this is unknown. We report that the MZ is enriched in “fetal-type” B cell receptors lacking N regions (N(−)). Mixed bone marrow (BM) chimeras, made with adult terminal deoxynucleotidyl transferase (TdT)(+/+) and TdT(−/−) donor cells, demonstrate preferential repertoire-based selection of N(−) B cells into the MZ. Reconstitution of irradiated mice with adult TdT(+/+) BM reveals that the MZ can replenish N(−) B cells in adult life via repertoire-based selection and suggest the possibility of a TdT-deficient precursor population in the adult BM. The mixed chimera data also suggest repertoire-based bifurcations into distinct BM and splenic maturation pathways, with mature “recirculating” BM B cells showing a very strong preference for N(+) complementarity-determining region (CDR) 3 compared with follicular B cells. Because the T1 and MZ compartments are both the most enriched for N(−) H-CDR3, we propose a novel direct T1→MZ pathway and identify a potential T1–MZ precursor intermediate. We demonstrate progressive but discontinuous repertoire-based selection throughout B cell development supporting multiple branchpoints and pathways in B cell development. Multiple differentiation routes leading to MZ development may contribute to the reported functional heterogeneity of the MZ compartment. The Rockefeller University Press 2008-09-01 /pmc/articles/PMC2526199/ /pubmed/18710933 http://dx.doi.org/10.1084/jem.20080559 Text en © 2008 Carey et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Articles Carey, John B. Moffatt-Blue, Chantelle S. Watson, Lisa C. Gavin, Amanda L. Feeney, Ann J. Repertoire-based selection into the marginal zone compartment during B cell development |
title | Repertoire-based selection into the marginal zone compartment during B cell development |
title_full | Repertoire-based selection into the marginal zone compartment during B cell development |
title_fullStr | Repertoire-based selection into the marginal zone compartment during B cell development |
title_full_unstemmed | Repertoire-based selection into the marginal zone compartment during B cell development |
title_short | Repertoire-based selection into the marginal zone compartment during B cell development |
title_sort | repertoire-based selection into the marginal zone compartment during b cell development |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526199/ https://www.ncbi.nlm.nih.gov/pubmed/18710933 http://dx.doi.org/10.1084/jem.20080559 |
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