Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification

Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is still required for cDNA library preparation from single-cell quantities of RNA. We have designed a small...

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Detalles Bibliográficos
Autores principales: Tougan, Takahiro, Okuzaki, Daisuke, Nojima, Hiroshi
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528176/
https://www.ncbi.nlm.nih.gov/pubmed/18603591
http://dx.doi.org/10.1093/nar/gkn420

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2528176/
https://www.ncbi.nlm.nih.gov/pubmed/18603591
http://dx.doi.org/10.1093/nar/gkn420

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