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Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi

BACKGROUND: The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion of coding...

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Detalles Bibliográficos
Autores principales: Frandsen, Rasmus JN, Andersson, Jens A, Kristensen, Matilde B, Giese, Henriette
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2533011/
https://www.ncbi.nlm.nih.gov/pubmed/18673530
http://dx.doi.org/10.1186/1471-2199-9-70