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Efficient four fragment cloning for the construction of vectors for targeted gene replacement in filamentous fungi
BACKGROUND: The rapid increase in whole genome fungal sequence information allows large scale functional analyses of target genes. Efficient transformation methods to obtain site-directed gene replacement, targeted over-expression by promoter replacement, in-frame epitope tagging or fusion of coding...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2533011/ https://www.ncbi.nlm.nih.gov/pubmed/18673530 http://dx.doi.org/10.1186/1471-2199-9-70 |