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Phi29 polymerase based random amplification of viral RNA as an alternative to random RT-PCR

BACKGROUND: Phi29 polymerase based amplification methods provides amplified DNA with minimal changes in sequence and relative abundance for many biomedical applications. RNA virus detection using microarrays, however, can present a challenge because phi29 DNA polymerase cannot amplify RNA nor small...

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Detalles Bibliográficos
Autores principales: Berthet, Nicolas, Reinhardt, Anita K, Leclercq, India, van Ooyen, Sven, Batéjat, Christophe, Dickinson, Philip, Stamboliyska, Rayna, Old, Iain G, Kong, Katherine A, Dacheux, Laurent, Bourhy, Hervé, Kennedy, Giulia C, Korfhage, Christian, Cole, Stewart T, Manuguerra, Jean-Claude
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2535778/
https://www.ncbi.nlm.nih.gov/pubmed/18771595
http://dx.doi.org/10.1186/1471-2199-9-77