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Association between active genes occurs at nuclear speckles and is modulated by chromatin environment

Genes on different chromosomes can be spatially associated in the nucleus in several transcriptional and regulatory situations; however, the functional significance of such associations remains unclear. Using human erythropoiesis as a model, we show that five cotranscribed genes, which are found on...

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Autores principales: Brown, Jill M., Green, Joanne, das Neves, Ricardo Pires, Wallace, Helen A.C., Smith, Andrew J.H., Hughes, Jim, Gray, Nicki, Taylor, Steve, Wood, William G., Higgs, Douglas R., Iborra, Francisco J., Buckle, Veronica J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542471/
https://www.ncbi.nlm.nih.gov/pubmed/18809724
http://dx.doi.org/10.1083/jcb.200803174
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author Brown, Jill M.
Green, Joanne
das Neves, Ricardo Pires
Wallace, Helen A.C.
Smith, Andrew J.H.
Hughes, Jim
Gray, Nicki
Taylor, Steve
Wood, William G.
Higgs, Douglas R.
Iborra, Francisco J.
Buckle, Veronica J.
author_facet Brown, Jill M.
Green, Joanne
das Neves, Ricardo Pires
Wallace, Helen A.C.
Smith, Andrew J.H.
Hughes, Jim
Gray, Nicki
Taylor, Steve
Wood, William G.
Higgs, Douglas R.
Iborra, Francisco J.
Buckle, Veronica J.
author_sort Brown, Jill M.
collection PubMed
description Genes on different chromosomes can be spatially associated in the nucleus in several transcriptional and regulatory situations; however, the functional significance of such associations remains unclear. Using human erythropoiesis as a model, we show that five cotranscribed genes, which are found on four different chromosomes, associate with each other at significant but variable frequencies. Those genes most frequently in association lie in decondensed stretches of chromatin. By replacing the mouse α-globin gene cluster in situ with its human counterpart, we demonstrate a direct effect of the regional chromatin environment on the frequency of association, whereas nascent transcription from the human α-globin gene appears unaffected. We see no evidence that cotranscribed erythroid genes associate at shared transcription foci, but we do see stochastic clustering of active genes around common nuclear SC35-enriched speckles (hence the apparent nonrandom association between genes). Thus, association between active genes may result from their location on decondensed chromatin that enables clustering around common nuclear speckles.
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spelling pubmed-25424712009-03-22 Association between active genes occurs at nuclear speckles and is modulated by chromatin environment Brown, Jill M. Green, Joanne das Neves, Ricardo Pires Wallace, Helen A.C. Smith, Andrew J.H. Hughes, Jim Gray, Nicki Taylor, Steve Wood, William G. Higgs, Douglas R. Iborra, Francisco J. Buckle, Veronica J. J Cell Biol Research Articles Genes on different chromosomes can be spatially associated in the nucleus in several transcriptional and regulatory situations; however, the functional significance of such associations remains unclear. Using human erythropoiesis as a model, we show that five cotranscribed genes, which are found on four different chromosomes, associate with each other at significant but variable frequencies. Those genes most frequently in association lie in decondensed stretches of chromatin. By replacing the mouse α-globin gene cluster in situ with its human counterpart, we demonstrate a direct effect of the regional chromatin environment on the frequency of association, whereas nascent transcription from the human α-globin gene appears unaffected. We see no evidence that cotranscribed erythroid genes associate at shared transcription foci, but we do see stochastic clustering of active genes around common nuclear SC35-enriched speckles (hence the apparent nonrandom association between genes). Thus, association between active genes may result from their location on decondensed chromatin that enables clustering around common nuclear speckles. The Rockefeller University Press 2008-09-22 /pmc/articles/PMC2542471/ /pubmed/18809724 http://dx.doi.org/10.1083/jcb.200803174 Text en © 2008 Brown et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Brown, Jill M.
Green, Joanne
das Neves, Ricardo Pires
Wallace, Helen A.C.
Smith, Andrew J.H.
Hughes, Jim
Gray, Nicki
Taylor, Steve
Wood, William G.
Higgs, Douglas R.
Iborra, Francisco J.
Buckle, Veronica J.
Association between active genes occurs at nuclear speckles and is modulated by chromatin environment
title Association between active genes occurs at nuclear speckles and is modulated by chromatin environment
title_full Association between active genes occurs at nuclear speckles and is modulated by chromatin environment
title_fullStr Association between active genes occurs at nuclear speckles and is modulated by chromatin environment
title_full_unstemmed Association between active genes occurs at nuclear speckles and is modulated by chromatin environment
title_short Association between active genes occurs at nuclear speckles and is modulated by chromatin environment
title_sort association between active genes occurs at nuclear speckles and is modulated by chromatin environment
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2542471/
https://www.ncbi.nlm.nih.gov/pubmed/18809724
http://dx.doi.org/10.1083/jcb.200803174
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