Cargando…
Meiotic Regulation of TPX2 Protein Levels Governs Cell Cycle Progression in Mouse Oocytes
Formation of female gametes requires acentriolar spindle assembly during meiosis. Mitotic spindles organize from centrosomes and via local activation of the RanGTPase on chromosomes. Vertebrate oocytes present a RanGTP gradient centred on chromatin at all stages of meiotic maturation. However, this...
Autores principales: | , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2556383/ https://www.ncbi.nlm.nih.gov/pubmed/18833336 http://dx.doi.org/10.1371/journal.pone.0003338 |
_version_ | 1782159566593589248 |
---|---|
author | Brunet, Stéphane Dumont, Julien Lee, Karen W. Kinoshita, Kazuhisa Hikal, Pascale Gruss, Oliver J. Maro, Bernard Verlhac, Marie-Hélène |
author_facet | Brunet, Stéphane Dumont, Julien Lee, Karen W. Kinoshita, Kazuhisa Hikal, Pascale Gruss, Oliver J. Maro, Bernard Verlhac, Marie-Hélène |
author_sort | Brunet, Stéphane |
collection | PubMed |
description | Formation of female gametes requires acentriolar spindle assembly during meiosis. Mitotic spindles organize from centrosomes and via local activation of the RanGTPase on chromosomes. Vertebrate oocytes present a RanGTP gradient centred on chromatin at all stages of meiotic maturation. However, this gradient is dispensable for assembly of the first meiotic spindle. To understand this meiosis I peculiarity, we studied TPX2, a Ran target, in mouse oocytes. Strikingly, TPX2 activity is controlled at the protein level through its accumulation from meiosis I to II. By RNAi depletion and live imaging, we show that TPX2 is required for spindle assembly via two distinct functions. It controls microtubule assembly and spindle pole integrity via the phosphorylation of TACC3, a regulator of MTOCs activity. We show that meiotic spindle formation in vivo depends on the regulation of at least a target of Ran, TPX2, rather than on the regulation of the RanGTP gradient itself. |
format | Text |
id | pubmed-2556383 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-25563832008-10-03 Meiotic Regulation of TPX2 Protein Levels Governs Cell Cycle Progression in Mouse Oocytes Brunet, Stéphane Dumont, Julien Lee, Karen W. Kinoshita, Kazuhisa Hikal, Pascale Gruss, Oliver J. Maro, Bernard Verlhac, Marie-Hélène PLoS One Research Article Formation of female gametes requires acentriolar spindle assembly during meiosis. Mitotic spindles organize from centrosomes and via local activation of the RanGTPase on chromosomes. Vertebrate oocytes present a RanGTP gradient centred on chromatin at all stages of meiotic maturation. However, this gradient is dispensable for assembly of the first meiotic spindle. To understand this meiosis I peculiarity, we studied TPX2, a Ran target, in mouse oocytes. Strikingly, TPX2 activity is controlled at the protein level through its accumulation from meiosis I to II. By RNAi depletion and live imaging, we show that TPX2 is required for spindle assembly via two distinct functions. It controls microtubule assembly and spindle pole integrity via the phosphorylation of TACC3, a regulator of MTOCs activity. We show that meiotic spindle formation in vivo depends on the regulation of at least a target of Ran, TPX2, rather than on the regulation of the RanGTP gradient itself. Public Library of Science 2008-10-03 /pmc/articles/PMC2556383/ /pubmed/18833336 http://dx.doi.org/10.1371/journal.pone.0003338 Text en Brunet et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Brunet, Stéphane Dumont, Julien Lee, Karen W. Kinoshita, Kazuhisa Hikal, Pascale Gruss, Oliver J. Maro, Bernard Verlhac, Marie-Hélène Meiotic Regulation of TPX2 Protein Levels Governs Cell Cycle Progression in Mouse Oocytes |
title | Meiotic Regulation of TPX2 Protein Levels Governs Cell Cycle Progression in Mouse Oocytes |
title_full | Meiotic Regulation of TPX2 Protein Levels Governs Cell Cycle Progression in Mouse Oocytes |
title_fullStr | Meiotic Regulation of TPX2 Protein Levels Governs Cell Cycle Progression in Mouse Oocytes |
title_full_unstemmed | Meiotic Regulation of TPX2 Protein Levels Governs Cell Cycle Progression in Mouse Oocytes |
title_short | Meiotic Regulation of TPX2 Protein Levels Governs Cell Cycle Progression in Mouse Oocytes |
title_sort | meiotic regulation of tpx2 protein levels governs cell cycle progression in mouse oocytes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2556383/ https://www.ncbi.nlm.nih.gov/pubmed/18833336 http://dx.doi.org/10.1371/journal.pone.0003338 |
work_keys_str_mv | AT brunetstephane meioticregulationoftpx2proteinlevelsgovernscellcycleprogressioninmouseoocytes AT dumontjulien meioticregulationoftpx2proteinlevelsgovernscellcycleprogressioninmouseoocytes AT leekarenw meioticregulationoftpx2proteinlevelsgovernscellcycleprogressioninmouseoocytes AT kinoshitakazuhisa meioticregulationoftpx2proteinlevelsgovernscellcycleprogressioninmouseoocytes AT hikalpascale meioticregulationoftpx2proteinlevelsgovernscellcycleprogressioninmouseoocytes AT grussoliverj meioticregulationoftpx2proteinlevelsgovernscellcycleprogressioninmouseoocytes AT marobernard meioticregulationoftpx2proteinlevelsgovernscellcycleprogressioninmouseoocytes AT verlhacmariehelene meioticregulationoftpx2proteinlevelsgovernscellcycleprogressioninmouseoocytes |