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The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes
The accuracy of chromosome segregation is enhanced by the spindle assembly checkpoint (SAC). The SAC is thought to monitor two distinct events: attachment of kinetochores to microtubules and the stretch of the centromere between the sister kinetochores that arises only when the chromosome becomes pr...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557037/ https://www.ncbi.nlm.nih.gov/pubmed/18824563 http://dx.doi.org/10.1083/jcb.200801038 |
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author | O'Connell, Christopher B. Lončarek, Jadranka Hergert, Polla Kourtidis, Antonis Conklin, Douglas S. Khodjakov, Alexey |
author_facet | O'Connell, Christopher B. Lončarek, Jadranka Hergert, Polla Kourtidis, Antonis Conklin, Douglas S. Khodjakov, Alexey |
author_sort | O'Connell, Christopher B. |
collection | PubMed |
description | The accuracy of chromosome segregation is enhanced by the spindle assembly checkpoint (SAC). The SAC is thought to monitor two distinct events: attachment of kinetochores to microtubules and the stretch of the centromere between the sister kinetochores that arises only when the chromosome becomes properly bioriented. We examined human cells undergoing mitosis with unreplicated genomes (MUG). Kinetochores in these cells are not paired, which implies that the centromere cannot be stretched; however, cells progress through mitosis. A SAC is present during MUG as cells arrest in response to nocodazole, taxol, or monastrol treatments. Mad2 is recruited to unattached MUG kinetochores and released upon their attachment. In contrast, BubR1 remains on attached kinetochores and exhibits a level of phosphorylation consistent with the inability of MUG spindles to establish normal levels of centromere tension. Thus, kinetochore attachment to microtubules is sufficient to satisfy the SAC even in the absence of interkinetochore tension. |
format | Text |
id | pubmed-2557037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25570372009-04-06 The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes O'Connell, Christopher B. Lončarek, Jadranka Hergert, Polla Kourtidis, Antonis Conklin, Douglas S. Khodjakov, Alexey J Cell Biol Research Articles The accuracy of chromosome segregation is enhanced by the spindle assembly checkpoint (SAC). The SAC is thought to monitor two distinct events: attachment of kinetochores to microtubules and the stretch of the centromere between the sister kinetochores that arises only when the chromosome becomes properly bioriented. We examined human cells undergoing mitosis with unreplicated genomes (MUG). Kinetochores in these cells are not paired, which implies that the centromere cannot be stretched; however, cells progress through mitosis. A SAC is present during MUG as cells arrest in response to nocodazole, taxol, or monastrol treatments. Mad2 is recruited to unattached MUG kinetochores and released upon their attachment. In contrast, BubR1 remains on attached kinetochores and exhibits a level of phosphorylation consistent with the inability of MUG spindles to establish normal levels of centromere tension. Thus, kinetochore attachment to microtubules is sufficient to satisfy the SAC even in the absence of interkinetochore tension. The Rockefeller University Press 2008-10-06 /pmc/articles/PMC2557037/ /pubmed/18824563 http://dx.doi.org/10.1083/jcb.200801038 Text en © 2008 O'Connell et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles O'Connell, Christopher B. Lončarek, Jadranka Hergert, Polla Kourtidis, Antonis Conklin, Douglas S. Khodjakov, Alexey The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes |
title | The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes |
title_full | The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes |
title_fullStr | The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes |
title_full_unstemmed | The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes |
title_short | The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes |
title_sort | spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557037/ https://www.ncbi.nlm.nih.gov/pubmed/18824563 http://dx.doi.org/10.1083/jcb.200801038 |
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