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The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes

The accuracy of chromosome segregation is enhanced by the spindle assembly checkpoint (SAC). The SAC is thought to monitor two distinct events: attachment of kinetochores to microtubules and the stretch of the centromere between the sister kinetochores that arises only when the chromosome becomes pr...

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Autores principales: O'Connell, Christopher B., Lončarek, Jadranka, Hergert, Polla, Kourtidis, Antonis, Conklin, Douglas S., Khodjakov, Alexey
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557037/
https://www.ncbi.nlm.nih.gov/pubmed/18824563
http://dx.doi.org/10.1083/jcb.200801038
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author O'Connell, Christopher B.
Lončarek, Jadranka
Hergert, Polla
Kourtidis, Antonis
Conklin, Douglas S.
Khodjakov, Alexey
author_facet O'Connell, Christopher B.
Lončarek, Jadranka
Hergert, Polla
Kourtidis, Antonis
Conklin, Douglas S.
Khodjakov, Alexey
author_sort O'Connell, Christopher B.
collection PubMed
description The accuracy of chromosome segregation is enhanced by the spindle assembly checkpoint (SAC). The SAC is thought to monitor two distinct events: attachment of kinetochores to microtubules and the stretch of the centromere between the sister kinetochores that arises only when the chromosome becomes properly bioriented. We examined human cells undergoing mitosis with unreplicated genomes (MUG). Kinetochores in these cells are not paired, which implies that the centromere cannot be stretched; however, cells progress through mitosis. A SAC is present during MUG as cells arrest in response to nocodazole, taxol, or monastrol treatments. Mad2 is recruited to unattached MUG kinetochores and released upon their attachment. In contrast, BubR1 remains on attached kinetochores and exhibits a level of phosphorylation consistent with the inability of MUG spindles to establish normal levels of centromere tension. Thus, kinetochore attachment to microtubules is sufficient to satisfy the SAC even in the absence of interkinetochore tension.
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spelling pubmed-25570372009-04-06 The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes O'Connell, Christopher B. Lončarek, Jadranka Hergert, Polla Kourtidis, Antonis Conklin, Douglas S. Khodjakov, Alexey J Cell Biol Research Articles The accuracy of chromosome segregation is enhanced by the spindle assembly checkpoint (SAC). The SAC is thought to monitor two distinct events: attachment of kinetochores to microtubules and the stretch of the centromere between the sister kinetochores that arises only when the chromosome becomes properly bioriented. We examined human cells undergoing mitosis with unreplicated genomes (MUG). Kinetochores in these cells are not paired, which implies that the centromere cannot be stretched; however, cells progress through mitosis. A SAC is present during MUG as cells arrest in response to nocodazole, taxol, or monastrol treatments. Mad2 is recruited to unattached MUG kinetochores and released upon their attachment. In contrast, BubR1 remains on attached kinetochores and exhibits a level of phosphorylation consistent with the inability of MUG spindles to establish normal levels of centromere tension. Thus, kinetochore attachment to microtubules is sufficient to satisfy the SAC even in the absence of interkinetochore tension. The Rockefeller University Press 2008-10-06 /pmc/articles/PMC2557037/ /pubmed/18824563 http://dx.doi.org/10.1083/jcb.200801038 Text en © 2008 O'Connell et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
O'Connell, Christopher B.
Lončarek, Jadranka
Hergert, Polla
Kourtidis, Antonis
Conklin, Douglas S.
Khodjakov, Alexey
The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes
title The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes
title_full The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes
title_fullStr The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes
title_full_unstemmed The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes
title_short The spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes
title_sort spindle assembly checkpoint is satisfied in the absence of interkinetochore tension during mitosis with unreplicated genomes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557037/
https://www.ncbi.nlm.nih.gov/pubmed/18824563
http://dx.doi.org/10.1083/jcb.200801038
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