Cargando…
Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation
Entry into mitosis is characterized by a dramatic remodeling of nuclear and cytoplasmic compartments. These changes are driven by cyclin-dependent kinase 1 (CDK1) activity, yet how cytoplasmic and nuclear CDK1 activities are coordinated is unclear. We injected cyclin B (CycB) into Drosophila melanog...
Autores principales: | , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557043/ https://www.ncbi.nlm.nih.gov/pubmed/18824564 http://dx.doi.org/10.1083/jcb.200801153 |
_version_ | 1782159625195356160 |
---|---|
author | Royou, Anne McCusker, Derek Kellogg, Douglas R. Sullivan, William |
author_facet | Royou, Anne McCusker, Derek Kellogg, Douglas R. Sullivan, William |
author_sort | Royou, Anne |
collection | PubMed |
description | Entry into mitosis is characterized by a dramatic remodeling of nuclear and cytoplasmic compartments. These changes are driven by cyclin-dependent kinase 1 (CDK1) activity, yet how cytoplasmic and nuclear CDK1 activities are coordinated is unclear. We injected cyclin B (CycB) into Drosophila melanogaster embryos during interphase of syncytial cycles and monitored effects on cytoplasmic and nuclear mitotic events. In untreated embryos or embryos arrested in interphase with a protein synthesis inhibitor, injection of CycB accelerates nuclear envelope breakdown and mitotic remodeling of the cytoskeleton. Upon activation of the Grapes(checkpoint kinase 1) (Grp(Chk1))-dependent S-phase checkpoint, increased levels of CycB drives cytoplasmic but not nuclear mitotic events. Grp(Chk1) prevents nuclear CDK1 activation by delaying CycB nuclear accumulation through Wee1-dependent and independent mechanisms. |
format | Text |
id | pubmed-2557043 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-25570432009-04-06 Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation Royou, Anne McCusker, Derek Kellogg, Douglas R. Sullivan, William J Cell Biol Research Articles Entry into mitosis is characterized by a dramatic remodeling of nuclear and cytoplasmic compartments. These changes are driven by cyclin-dependent kinase 1 (CDK1) activity, yet how cytoplasmic and nuclear CDK1 activities are coordinated is unclear. We injected cyclin B (CycB) into Drosophila melanogaster embryos during interphase of syncytial cycles and monitored effects on cytoplasmic and nuclear mitotic events. In untreated embryos or embryos arrested in interphase with a protein synthesis inhibitor, injection of CycB accelerates nuclear envelope breakdown and mitotic remodeling of the cytoskeleton. Upon activation of the Grapes(checkpoint kinase 1) (Grp(Chk1))-dependent S-phase checkpoint, increased levels of CycB drives cytoplasmic but not nuclear mitotic events. Grp(Chk1) prevents nuclear CDK1 activation by delaying CycB nuclear accumulation through Wee1-dependent and independent mechanisms. The Rockefeller University Press 2008-10-06 /pmc/articles/PMC2557043/ /pubmed/18824564 http://dx.doi.org/10.1083/jcb.200801153 Text en © 2008 Royou et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Royou, Anne McCusker, Derek Kellogg, Douglas R. Sullivan, William Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation |
title | Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation |
title_full | Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation |
title_fullStr | Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation |
title_full_unstemmed | Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation |
title_short | Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation |
title_sort | grapes(chk1) prevents nuclear cdk1 activation by delaying cyclin b nuclear accumulation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557043/ https://www.ncbi.nlm.nih.gov/pubmed/18824564 http://dx.doi.org/10.1083/jcb.200801153 |
work_keys_str_mv | AT royouanne grapeschk1preventsnuclearcdk1activationbydelayingcyclinbnuclearaccumulation AT mccuskerderek grapeschk1preventsnuclearcdk1activationbydelayingcyclinbnuclearaccumulation AT kelloggdouglasr grapeschk1preventsnuclearcdk1activationbydelayingcyclinbnuclearaccumulation AT sullivanwilliam grapeschk1preventsnuclearcdk1activationbydelayingcyclinbnuclearaccumulation |