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Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation

Entry into mitosis is characterized by a dramatic remodeling of nuclear and cytoplasmic compartments. These changes are driven by cyclin-dependent kinase 1 (CDK1) activity, yet how cytoplasmic and nuclear CDK1 activities are coordinated is unclear. We injected cyclin B (CycB) into Drosophila melanog...

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Detalles Bibliográficos
Autores principales: Royou, Anne, McCusker, Derek, Kellogg, Douglas R., Sullivan, William
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557043/
https://www.ncbi.nlm.nih.gov/pubmed/18824564
http://dx.doi.org/10.1083/jcb.200801153
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author Royou, Anne
McCusker, Derek
Kellogg, Douglas R.
Sullivan, William
author_facet Royou, Anne
McCusker, Derek
Kellogg, Douglas R.
Sullivan, William
author_sort Royou, Anne
collection PubMed
description Entry into mitosis is characterized by a dramatic remodeling of nuclear and cytoplasmic compartments. These changes are driven by cyclin-dependent kinase 1 (CDK1) activity, yet how cytoplasmic and nuclear CDK1 activities are coordinated is unclear. We injected cyclin B (CycB) into Drosophila melanogaster embryos during interphase of syncytial cycles and monitored effects on cytoplasmic and nuclear mitotic events. In untreated embryos or embryos arrested in interphase with a protein synthesis inhibitor, injection of CycB accelerates nuclear envelope breakdown and mitotic remodeling of the cytoskeleton. Upon activation of the Grapes(checkpoint kinase 1) (Grp(Chk1))-dependent S-phase checkpoint, increased levels of CycB drives cytoplasmic but not nuclear mitotic events. Grp(Chk1) prevents nuclear CDK1 activation by delaying CycB nuclear accumulation through Wee1-dependent and independent mechanisms.
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spelling pubmed-25570432009-04-06 Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation Royou, Anne McCusker, Derek Kellogg, Douglas R. Sullivan, William J Cell Biol Research Articles Entry into mitosis is characterized by a dramatic remodeling of nuclear and cytoplasmic compartments. These changes are driven by cyclin-dependent kinase 1 (CDK1) activity, yet how cytoplasmic and nuclear CDK1 activities are coordinated is unclear. We injected cyclin B (CycB) into Drosophila melanogaster embryos during interphase of syncytial cycles and monitored effects on cytoplasmic and nuclear mitotic events. In untreated embryos or embryos arrested in interphase with a protein synthesis inhibitor, injection of CycB accelerates nuclear envelope breakdown and mitotic remodeling of the cytoskeleton. Upon activation of the Grapes(checkpoint kinase 1) (Grp(Chk1))-dependent S-phase checkpoint, increased levels of CycB drives cytoplasmic but not nuclear mitotic events. Grp(Chk1) prevents nuclear CDK1 activation by delaying CycB nuclear accumulation through Wee1-dependent and independent mechanisms. The Rockefeller University Press 2008-10-06 /pmc/articles/PMC2557043/ /pubmed/18824564 http://dx.doi.org/10.1083/jcb.200801153 Text en © 2008 Royou et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Royou, Anne
McCusker, Derek
Kellogg, Douglas R.
Sullivan, William
Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation
title Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation
title_full Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation
title_fullStr Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation
title_full_unstemmed Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation
title_short Grapes(Chk1) prevents nuclear CDK1 activation by delaying cyclin B nuclear accumulation
title_sort grapes(chk1) prevents nuclear cdk1 activation by delaying cyclin b nuclear accumulation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2557043/
https://www.ncbi.nlm.nih.gov/pubmed/18824564
http://dx.doi.org/10.1083/jcb.200801153
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