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A High-Throughput Assay for siRNA-Based Circadian Screens in Human U2OS Cells

The advent of siRNA-based screens has revolutionized the efficiency by which functional components of biological processes are identified. A notable exception has been the field of mammalian circadian rhythms. Here, we outline a medium- to high-throughput siRNA-based approach that, in combination wi...

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Detalles Bibliográficos
Autores principales: Vollmers, Christopher, Panda, Satchidananda, DiTacchio, Luciano
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2565498/
https://www.ncbi.nlm.nih.gov/pubmed/18941634
http://dx.doi.org/10.1371/journal.pone.0003457