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Predicting the sensitivity and specificity of published real-time PCR assays

BACKGROUND: In recent years real-time PCR has become a leading technique for nucleic acid detection and quantification. These assays have the potential to greatly enhance efficiency in the clinical laboratory. Choice of primer and probe sequences is critical for accurate diagnosis in the clinic, yet...

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Detalles Bibliográficos
Autores principales: Lemmon, Gordon H, Gardner, Shea N
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566554/
https://www.ncbi.nlm.nih.gov/pubmed/18817537
http://dx.doi.org/10.1186/1476-0711-7-18