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14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers

Activation of the tumour suppressor p53 on DNA damage involves post-translational modification by phosphorylation and acetylation. Phosphorylation of certain residues is critical for p53 stabilization and plays an important role in DNA-binding activity. The 14-3-3 family of proteins activates the DN...

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Autores principales: Rajagopalan, Sridharan, Jaulent, Agnes M., Wells, Mark, Veprintsev, Dmitry B., Fersht, Alan R.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566891/
https://www.ncbi.nlm.nih.gov/pubmed/18812399
http://dx.doi.org/10.1093/nar/gkn598
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author Rajagopalan, Sridharan
Jaulent, Agnes M.
Wells, Mark
Veprintsev, Dmitry B.
Fersht, Alan R.
author_facet Rajagopalan, Sridharan
Jaulent, Agnes M.
Wells, Mark
Veprintsev, Dmitry B.
Fersht, Alan R.
author_sort Rajagopalan, Sridharan
collection PubMed
description Activation of the tumour suppressor p53 on DNA damage involves post-translational modification by phosphorylation and acetylation. Phosphorylation of certain residues is critical for p53 stabilization and plays an important role in DNA-binding activity. The 14-3-3 family of proteins activates the DNA-binding affinity of p53 upon stress by binding to a site in its intrinsically disordered C-terminal domain containing a phosphorylated serine at 378. We have screened various p53 C-terminal phosphorylated peptides for binding to two different isoforms of 14-3-3, ɛ and γ. We found that phosphorylation at either S366 or T387 caused even tighter binding to 14-3-3. We made by semi-synthesis a tetrameric construct comprised of the tetramerization plus C-terminal domains of p53 that was phosphorylated on S366, S378 and T387. It bound 10 times tighter than did the monomeric counterpart to dimeric 14-3-3. We showed indirectly from binding curves and directly from fluorescence-detection analytical ultracentrifugation that 14-3-3 enhanced the binding of sequence-specific DNA to p53 by causing p53 dimers to form tetramers at lower concentrations. If the in vitro data extrapolate to in vivo, then it is an attractive hypothesis that p53 activity may be subject to control by accessory proteins lowering its tetramer–dimer dissociation constant from its normal value of 120–150 nM.
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spelling pubmed-25668912008-10-17 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers Rajagopalan, Sridharan Jaulent, Agnes M. Wells, Mark Veprintsev, Dmitry B. Fersht, Alan R. Nucleic Acids Res Molecular Biology Activation of the tumour suppressor p53 on DNA damage involves post-translational modification by phosphorylation and acetylation. Phosphorylation of certain residues is critical for p53 stabilization and plays an important role in DNA-binding activity. The 14-3-3 family of proteins activates the DNA-binding affinity of p53 upon stress by binding to a site in its intrinsically disordered C-terminal domain containing a phosphorylated serine at 378. We have screened various p53 C-terminal phosphorylated peptides for binding to two different isoforms of 14-3-3, ɛ and γ. We found that phosphorylation at either S366 or T387 caused even tighter binding to 14-3-3. We made by semi-synthesis a tetrameric construct comprised of the tetramerization plus C-terminal domains of p53 that was phosphorylated on S366, S378 and T387. It bound 10 times tighter than did the monomeric counterpart to dimeric 14-3-3. We showed indirectly from binding curves and directly from fluorescence-detection analytical ultracentrifugation that 14-3-3 enhanced the binding of sequence-specific DNA to p53 by causing p53 dimers to form tetramers at lower concentrations. If the in vitro data extrapolate to in vivo, then it is an attractive hypothesis that p53 activity may be subject to control by accessory proteins lowering its tetramer–dimer dissociation constant from its normal value of 120–150 nM. Oxford University Press 2008-10 2008-09-23 /pmc/articles/PMC2566891/ /pubmed/18812399 http://dx.doi.org/10.1093/nar/gkn598 Text en © 2008 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
Rajagopalan, Sridharan
Jaulent, Agnes M.
Wells, Mark
Veprintsev, Dmitry B.
Fersht, Alan R.
14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers
title 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers
title_full 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers
title_fullStr 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers
title_full_unstemmed 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers
title_short 14-3-3 activation of DNA binding of p53 by enhancing its association into tetramers
title_sort 14-3-3 activation of dna binding of p53 by enhancing its association into tetramers
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2566891/
https://www.ncbi.nlm.nih.gov/pubmed/18812399
http://dx.doi.org/10.1093/nar/gkn598
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