Display of E. coli Alkaline Phosphatase pIII or pVIII Fusions on Phagemid Surfaces Reveals Monovalent Decoration with Active Molecules

Active alkaline phosphatase of Escherichia coli (PhoA, EC 3.1.3.1) was displayed via the leucine zipper element of the Jun-Fos heterodimer on the surface of filamentous phage and the kinetic parameters K(m) and k(cat) were determined. The phoA gene was cloned downstream of fos while jun was inserted...

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Detalles Bibliográficos
Autores principales: Weichel, Michael, Jaussi, Rolf, Rhyner, Claudio, Crameri, Reto
Formato: Texto
Lenguaje:English
Publicado: Bentham Open 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570559/
https://www.ncbi.nlm.nih.gov/pubmed/18949073
http://dx.doi.org/10.2174/1874091X00802010038

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570559/
https://www.ncbi.nlm.nih.gov/pubmed/18949073
http://dx.doi.org/10.2174/1874091X00802010038

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