One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester

BACKGROUND: Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation reactions to introduce synthetic groups or to immobilize proteins on surfaces and...

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Detalles Bibliográficos
Autores principales: Bastings, Maartje MC, van Baal, Ingrid, Meijer, EW, Merkx, Maarten
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2008
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570673/
https://www.ncbi.nlm.nih.gov/pubmed/18828922
http://dx.doi.org/10.1186/1472-6750-8-76

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570673/
https://www.ncbi.nlm.nih.gov/pubmed/18828922
http://dx.doi.org/10.1186/1472-6750-8-76

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