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One-step refolding and purification of disulfide-containing proteins with a C-terminal MESNA thioester
BACKGROUND: Expression systems based on self-cleavable intein domains allow the generation of recombinant proteins with a C-terminal thioester. This uniquely reactive C-terminus can be used in native chemical ligation reactions to introduce synthetic groups or to immobilize proteins on surfaces and...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2570673/ https://www.ncbi.nlm.nih.gov/pubmed/18828922 http://dx.doi.org/10.1186/1472-6750-8-76 |