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Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development

Notch signaling regulates lineage decisions at multiple stages of lymphocyte development, and Notch activation requires the endocytosis of Notch ligands in the signal-sending cells. Four E3 ubiquitin ligases, Mind bomb (Mib) 1, Mib2, Neuralized (Neur) 1, and Neur2, regulate the Notch ligands to acti...

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Autores principales: Song, Ran, Kim, Young-Woong, Koo, Bon-Kyoung, Jeong, Hyun-Woo, Yoon, Mi-Jeong, Yoon, Ki-Jun, Jun, Dong-Jae, Im, Sun-Kyoung, Shin, Juhee, Kong, Myoung-Phil, Kim, Kyong-Tai, Yoon, Keejung, Kong, Young-Yun
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2571928/
https://www.ncbi.nlm.nih.gov/pubmed/18824586
http://dx.doi.org/10.1084/jem.20081344
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author Song, Ran
Kim, Young-Woong
Koo, Bon-Kyoung
Jeong, Hyun-Woo
Yoon, Mi-Jeong
Yoon, Ki-Jun
Jun, Dong-Jae
Im, Sun-Kyoung
Shin, Juhee
Kong, Myoung-Phil
Kim, Kyong-Tai
Yoon, Keejung
Kong, Young-Yun
author_facet Song, Ran
Kim, Young-Woong
Koo, Bon-Kyoung
Jeong, Hyun-Woo
Yoon, Mi-Jeong
Yoon, Ki-Jun
Jun, Dong-Jae
Im, Sun-Kyoung
Shin, Juhee
Kong, Myoung-Phil
Kim, Kyong-Tai
Yoon, Keejung
Kong, Young-Yun
author_sort Song, Ran
collection PubMed
description Notch signaling regulates lineage decisions at multiple stages of lymphocyte development, and Notch activation requires the endocytosis of Notch ligands in the signal-sending cells. Four E3 ubiquitin ligases, Mind bomb (Mib) 1, Mib2, Neuralized (Neur) 1, and Neur2, regulate the Notch ligands to activate Notch signaling, but their roles in lymphocyte development have not been defined. We show that Mib1 regulates T and marginal zone B (MZB) cell development in the lymphopoietic niches. Inactivation of the Mib1 gene, but not the other E3 ligases, Mib2, Neur1, and Neur2, abrogated T and MZB cell development. Reciprocal bone marrow (BM) transplantation experiments revealed that Mib1 in the thymic and splenic niches is essential for T and MZB cell development. Interestingly, when BM cells from transgenic Notch reporter mice were transplanted into Mib1-null mice, the Notch signaling was abolished in the double-negative thymocytes. In addition, the endocytosis of Dll1 was impaired in the Mib1-null microenvironment. Moreover, the block in T cell development and the failure of Dll1 endocytosis were also observed in coculture system by Mib1 knockdown. Our study reveals that Mib1 is the essential E3 ligase in T and MZB cell development, through the regulation of Notch ligands in the thymic and splenic microenvironments.
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spelling pubmed-25719282009-04-27 Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development Song, Ran Kim, Young-Woong Koo, Bon-Kyoung Jeong, Hyun-Woo Yoon, Mi-Jeong Yoon, Ki-Jun Jun, Dong-Jae Im, Sun-Kyoung Shin, Juhee Kong, Myoung-Phil Kim, Kyong-Tai Yoon, Keejung Kong, Young-Yun J Exp Med Articles Notch signaling regulates lineage decisions at multiple stages of lymphocyte development, and Notch activation requires the endocytosis of Notch ligands in the signal-sending cells. Four E3 ubiquitin ligases, Mind bomb (Mib) 1, Mib2, Neuralized (Neur) 1, and Neur2, regulate the Notch ligands to activate Notch signaling, but their roles in lymphocyte development have not been defined. We show that Mib1 regulates T and marginal zone B (MZB) cell development in the lymphopoietic niches. Inactivation of the Mib1 gene, but not the other E3 ligases, Mib2, Neur1, and Neur2, abrogated T and MZB cell development. Reciprocal bone marrow (BM) transplantation experiments revealed that Mib1 in the thymic and splenic niches is essential for T and MZB cell development. Interestingly, when BM cells from transgenic Notch reporter mice were transplanted into Mib1-null mice, the Notch signaling was abolished in the double-negative thymocytes. In addition, the endocytosis of Dll1 was impaired in the Mib1-null microenvironment. Moreover, the block in T cell development and the failure of Dll1 endocytosis were also observed in coculture system by Mib1 knockdown. Our study reveals that Mib1 is the essential E3 ligase in T and MZB cell development, through the regulation of Notch ligands in the thymic and splenic microenvironments. The Rockefeller University Press 2008-10-27 /pmc/articles/PMC2571928/ /pubmed/18824586 http://dx.doi.org/10.1084/jem.20081344 Text en © 2008 Song et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Articles
Song, Ran
Kim, Young-Woong
Koo, Bon-Kyoung
Jeong, Hyun-Woo
Yoon, Mi-Jeong
Yoon, Ki-Jun
Jun, Dong-Jae
Im, Sun-Kyoung
Shin, Juhee
Kong, Myoung-Phil
Kim, Kyong-Tai
Yoon, Keejung
Kong, Young-Yun
Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development
title Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development
title_full Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development
title_fullStr Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development
title_full_unstemmed Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development
title_short Mind bomb 1 in the lymphopoietic niches is essential for T and marginal zone B cell development
title_sort mind bomb 1 in the lymphopoietic niches is essential for t and marginal zone b cell development
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2571928/
https://www.ncbi.nlm.nih.gov/pubmed/18824586
http://dx.doi.org/10.1084/jem.20081344
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